银屑病病人外周血单核细胞TLR2基因表达及血清ASO和IL8水平变化_医学论文.docVIP

银屑病病人外周血单核细胞TLR2基因表达及血清ASO和IL8水平变化_医学论文 银屑病病人外周血单核细胞TLR2基因表达及血清ASO和IL8水平变化_医学论文 【摘要】 目的 观察寻常型银屑病病人外周血单核细胞Toll样受体2（TLR2）mRNA表达及血清抗链球菌溶血素“O”（ASO）和白细胞介素（IL）水平的变化,探讨其与银屑病发病的关系。方法 采用RT方法检测银屑病病人(银屑病组)外周血单核细胞TLR2 mRNA表达,采用胶乳凝集法检测血清ASO水平,采用ELISA法检测血清IL水平。以我院体检健康者30例作为对照(正常对照组)。结果 银屑病组TLR2 mRNA表达明显高于正常对照组（t=5.541,P＜0.01）；银屑病组点滴型与斑块型TLR2 mRNA表达比较无显著性差异（t=0.318,P＞0.05）。银屑病组血清ASO水平明显高于正常对照组（χ2=8.604,P＜0.01）；且点滴型病人明显高于斑块型病人（χ2=9.780,P＜0.01）。银屑病组血清IL水平显著高于正常对照组（t=28.463,P＜0.01）；点滴型与斑块型病人之间比较无显著性差异（t=0.660,P＞0.05）。点滴型病人ASO阳性者TLR2 mRNA表达水平高于ASO阴性者,差异有显著性（t=2.407,P＜0.05）；银屑病病人外周血TLR2 mRNA表达与IL水平呈正相关（r=0.637,P＜0.01）。结论 TLR2可能通过识别链球菌等入侵病原微生物启动机体免疫应答,介导细胞因子IL等的产生,参与银屑病皮损的发生和发展。 【关键词】 银屑病 Toll样受体2 抗链球菌溶血素 白细胞介素8 ［ABSTRACT］ObjectiveTo investigate the expression of tollonocytes of psoriatic patients’ blood and the serum levels of interleukin8 (IL8) and antistreptolysin O (ASO), and to explore their relevance to pathogenesis of psoriasis.MethodsExpression of TLR2 mRNA were detected by reverse transcription polymerase chain reaction (RTPCR) method serum levels of ASO and IL8 were detected by latex agglutination assay and enzyme linked immunosorbent assay (ELISA) respectively. ResultsExpression of TLR2 mRNA of the patients was higher than that of the controls (t=5.541,P＜0.01). There was no significant difference in the expression of TLR2 mRNA between guttate and plaque psoriatic patients (t=0.318,P＞0.05). The positive rate of ASO in the patients was higher than that of the controls (χ2=8.604,P＜0.01) and the ASO positive rate in guttate patients was higher than that in plaque psoriatic patients (χ2=9.780,P＜0.01). Level of IL＜0.01) there was no significant difference between guttate and plaque psoriatic patients (t=0.660,P＞0.05). The expression of TLR2 mRNA in psoriatic patients with positive ASO was higher than that in patients with negetive ASO (t=2.407,P＜0.05) the expressions of TLR2 mRNA and IL8 in psoriatic patients had a positive correlation (r=0.637, P＜0.01). ConclusionTLR2 may