预变性神经移植促进神经再生的动物实验_医学论文.docVIP

预变性神经移植促进神经再生的动物实验_医学论文 预变性神经移植促进神经再生的动物实验_医学论文 【关键词】 神经再生 　　关键词: 神经再生神经移植动物，实验 　　摘 要:目的 通过兔腓总神经不同时限预变性神经移植，从组织学、免疫组织细胞化学、电生理、超微结构等多方面对神经功能恢复进行探讨. 方法 家兔42只分为A，B，C，D，E，F，G7组:A，B，C，D，E，F组分别为预变性0，4，7，14，21和28d移植组，G组动物双侧腓总神经分别作新鲜神经移植和预变性神经移植. 结果 2wk预变性神经移植与新鲜神经移植相比可显著降低轴突再生起初延迟期（由3.04～0.04d），提高神经生长速度（由1.73～2.59mm#12539d-1 ），促进N-MCV（由25.14～29.21m#12539s-1 ），增进轴突通过率（由81.2%～91%）.组织学观察预变性2wk再生轴突比新鲜神经移植组成熟，髓鞘化程度高而且早. 结论 预变性2wk神经移植无论在质和量上均优于新鲜神经移植，其次是预变性1wk神经移植组.预变性4d和3wk也有增强神经再生的作用. 　　Keywords:nerve graftnerve regenerationanimals，labora-tory 　　Abstract:AIM To explore the effect of predegenerated nerve grafting on nerve regeneration.METHODS We re-viewed nerve regeneration of different period predegenerated graftting through histologicalimmunocytochemicalelectron micoscopic and electrophysiological studies.The rabbits were divided into7experimental groups for different periods（0，4，7，14，21，28d）of nerve graftting.In A，B，C，D，E，F groups，both sides of the rabbit peroneal nerve were cut and allowed to undergo predegneration for0，4，7，14，21，28daysa10mm segment was used as an autologous nerve graft and transposed to a10mm long nerve defect on the con-tralateral side.In G group，the left side（Predegenerated nerve graft for14d）was compared to the right side（Fresh nerve graft）.RESULTS Graftting predegenerated for14days shortened significantly the initial delay period of axonal regeneration（3.04～0.04d），accelerated the axonal grow rate（1.73～2.59mm#12539d-1 ），increased the quantity of re-generated axon，and raised nerve muscle conduct velocity（25.14～29.21m#12539s-1 ）than fresh nerve grafts，also ma-tured earlier and better than ones for fresh nerve graft.CON┐CLUSION Grafts degenerated for14days can enhance sig-nificantly nerve regeneration than fresh nerve grafts，then grafts degenerated for7days.Grafts degenerated for4and21days can also enhance axonal regeneration lightly.It is suggested that grafts degenerated can provide better opportu-nity for axonal growth. 　　0 引言 　　1977年，Robe