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葡聚糖水合二激酶的生物学功能及其应用.pdf
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Journal of Zhejiang University ( Ag ric . Life Sci .)
http :/ / w w w .journals .zju .edu .cn/ ag r
Email :zdx bnsb zju .edu .cn DOI : ./ j .issn . . . .
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lucanw ater dikinase ,GWD)
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Biological function and application of glucan water dikinase : a review . Journal of Zhejiang U niversity
Ag ric . Life Sci .) , , :
( ( )
Sun Xiao , Bao Jinsong I ns titute of N uc lear A g r icultural Sc iences ,Zhej iang Univ e rs ity , H angz hou 310029 ,
China
Summary Starch phosphorylation is a general phenomenon existing naturally in the plant kingdom . T he
reaction of phosphorylation catalyzed by the glucan w ater dikinase ( GWD ) is a necessary process during the
degradation of the transitory starch in plant . In this review , w e summarized the prog ress made on GWD f rom the
view of its structure , biological functions and its interactions w ith other enzymes involved in starch metabolism .
We also discussed its potential application in starch processing industries to provide environment f riendly methods
for starch modification through genetic engineering . Potato GWD gene ( S tG W D) encodes a pro tein of amino
acid residues w ith a calculated molecular mass of about ku for the entire coding sequence . From the N terminal
to Cterminal of the mature StGWD protein , there is a chloroplast transit peptide domain , tw o carbohydrate
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