原发性高血压患者血浆ADMA浓度外周循环内皮祖细胞数量的变化_临床医学论文.docVIP

原发性高血压患者血浆ADMA浓度外周循环内皮祖细胞数量的变化_临床医学论文 原发性高血压患者血浆ADMA浓度外周循环内皮祖细胞数量的变化_临床医学论文 【摘要】 目的 探讨原发性高血压（EH）患者血浆非对称性二甲基精氨酸(ADMA)浓度、外周循环内皮祖细胞(EPCs)数量的变化及两者之间的关系。方法 选取停止服用降压药至少2周以上并排除冠状动脉粥样硬化性心脏病、糖尿病的原发性高血压患者46例，对照组25例为健康体检者。采用反相高效液相色谱法（RP-HPLC）测定血浆ADMA含量，流式细胞术分析外周循环EPCs。结果 EH组血浆ADMA浓度［(0.363±0.029)μg/ml］显著高于对照组［(0.319±0.022)μg/ml］(0.01)。EH组外周循环EPCs数量［(0.368±0.121)%］显著低于对照组［(0.588±0.139)%］（0.01）。血浆ADMA浓度与外周循环EPCs数量呈负相关关系(r=-0.706,0.01)。结论 EH患者血浆ADMA浓度升高，外周循环EPCs数量减少，两者之间呈负相关关系。EH患者循环EPCs数量下降部分原因可能是由于血浆ADMA水平升高所致。 【关键词】 原发性高血压 非对称性二甲基精氨酸 内皮祖细胞 【Abstract】 Objective To investigate the change of concentration of plasma asymmetric dimethylarginine(ADMA) and number of circulating endothelial progenitor cells（EPCs）in essential hypertensive patients(EH). Methods 46 EH patients and 25 healthy controls were recruited. Plasma ADMA were measured by reversed-phase high performance liquid chromatogram(RP-HPLC). Circulating EPCs was determinated by flow cytometry assay. Results The concentration of plasma ADMA in EH patients was higher than that in control ［(0.363±0.029)μg/ml vs (0.319±0.022)μg/ml, 0.01］. The number of circulating EPCs in EH patients was lower than that in control ［(0.368±0.121)% vs(0.588±0.139)%, 0.01］. The concentration of plasma ADMA was correlated inversely with the number of circulating EPCs in EH group(r=-0.706, 0.01). Conclusions The plasma ADMA concentration is increased and the number of circulating EPCs is decreased in EH patients. The concentration of plasma ADMA is correlated inversely with the number of circulating EPCs. The number of circulating EPCs is partly decreased because of the elevation of the plasma ADMA concentration. 【Key words】 Essential hypertension Asymmetric dimethylarginine Endothelial progenitor cells 血管内皮功能损伤是原发性高血压(EH)始动机制之一。非对称性二甲基精氨酸(Asymmetric Dimethylarginine, ADMA)是一个内源性一氧化氮合成酶(NOS)抑制剂，它可能通过抑制NO的产生，影响血管内皮功能［１］。内皮祖细胞(EPCs)是一类能分化为血管内皮细胞的前体细胞，来源于骨髓，在一定状态下能够动员到外周循环，分化为成熟内皮细胞并参与血管修复或形成。冠心病患者血浆ADMA水平和外周循环EPCs存在的关系已有报道［2］，而原发性高血压患者血浆ADMA和外周循环EP