培哚普利对U937泡沫细胞血管内皮生长因子表达的影响_临床医学论文.docVIP

培哚普利对U937泡沫细胞血管内皮生长因子表达的影响_临床医学论文 培哚普利对U937泡沫细胞血管内皮生长因子表达的影响_临床医学论文 【摘要】 目的：研究培哚普利对氧化修饰的低密度脂蛋白(ox诱导的U937泡沫细胞血管内皮生长因子(VEGF)mRNA、蛋白表达的影响。方法: U937细胞与80 μg/ｍL ox孵育48 h，建立U937泡沫细胞模型，以不同浓度的培哚普利（0.01、0.10、1.00 μmol/L）预处理U937细胞24 h再加入80 μg/ｍL 的ox孵育48 h，通过逆转录聚合酶链式扩增反应(RT和酶联免疫吸附试验(ELISA)分别检测U937细胞VEGF mRNA和蛋白的表达情况。 结果： U937泡沫细胞组VEGF mRNA的表达较U937细胞对照组明显增加［（2.371±0.253）vs（0.954±0.245）（0.01）］，培哚普利干预后，随着浓度（0.01、0.10、1.00 μmol/L）的增加，U937泡沫细胞的VEGF mRNA表达水平呈浓度依赖性下降［（2.168±0.270）vs（1.533±0.248）vs（1.022±0.189）（0.01）］；U937泡沫细胞组VEGF蛋白表达较U937细胞对照组明显增加［（1 804.18±177.59） pg/mL vs （716.19±60.82） pg/mL（ 0.01）］,培哚普利干预后，随着浓度（0.01、0.10、1.00 μmol/L）的增加，U937泡沫细胞VEGF蛋白表达呈浓度依赖性降低（1 601.46±154.68） pg/mL vs （1 377.09±110.36） pg/mL vs （1 017.89±147.18） pg/mL（0.01）]。结论: 培哚普利能够浓度依赖性地下调ox诱导的U937泡沫细胞VEGF mRNA、蛋白的表达。 【关键词】 培哚普利；泡沫细胞；血管内皮生长因子 ［ABSTRACT］ Objective: To study the expression of vascular endothelial growth factor (VEGF) induced by oxidized low density liprotein (oxNA in the U937 foam cells.Methods: The U937 cells were incubated with oxLDL 80 μg/mL for 48 h and a macrophagederived foam cell model was established. The medium was pretreated with perindopril (0.01、0.1、1 μmol/L) for 24 h , incubated with oxL for 48 h.Reverse transcriptionpolymerase chain reaction(RTPCR) and enzymelinked immuneosorbent assay (ELISA) were applied to determine the expression of VEGFprotein and mRNA in U937 cells and treated U937 cells.Results: VEGFmRNA levels in U937 cells after incubation with oxLDL 80 μg/mL increased significantly compared with control cells(foam cells vs control cells, 2.371±0.253 vs 0.954±0.245, 0.01).After treated with perindopril(0.01、0.10、1.00 μmol/L), VEGF protein levels were concentrationttenuated(foam cells vs perindopril treated cells, 2.371±0.253 vs 2.168±0.270、1.533±0.248、1.022±0.189, 0.01). VEGF protein levels in the medium after incubation with oxcells , 1 804.18±177.59 pg/mL vs 716.19±60.82 pg/mL, 0.01).After treated with perindopril(0.01, 0.10, 1.00 μmol/L), VEGF protein levels