外源核苷酸多代发育安全性评价_临床医学论文.docVIP

外源核苷酸多代发育安全性评价_临床医学论文 外源核苷酸多代发育安全性评价_临床医学论文 作者：马奕 徐琳琳 许雅君 李勇 【摘要】 目的 通过三代繁殖试验，观察外源性核苷酸对于啮齿类大鼠生育能力和后代生理、神经指标发育的影响，从而做出发育安全性评价。 方法 SD大鼠(F0代)断乳后，喂核苷酸，含量分别为0、0.01 %、0.04 %、0.16 %、0.64 %、1.28 %的受试物饲料90 d后雌、雄1∶1交配，所产仔鼠为Fl;Fl断乳后喂养90 d后进行二代繁殖试验。重复上述实验方法直到多代繁殖实验结束，F3代出生;期间观察各亲代大鼠孕期、分娩、哺乳期情况和各子代哺乳期情况、生理发育指标、神经反射指标。 结果 外源补充核苷酸并未使亲代大鼠的孕期、分娩、哺乳期以及子代大鼠的哺乳期、生理和神经发育反射出现异常情况。 结论 在本实验剂量下，补充外源补充核苷酸对于多代大鼠的生殖功能和胚胎期、胎儿期以及哺乳期的发育是安全的。 【关键词】 外源核苷酸;发育;安全性 　　【Abstract】 Objective To investigate the effect of exogenous nucleotides on fertility in parents rats and the indexes of physiological and neural development in newborn rats over the three- reproductive experiment, and evaluate the safety on development.MethodsWeanling rats (F0) were fed to exogenous nucleotides at the dose of 0, 0.01 %, 0.04 %, 0.16 %, 0.64 %, 1.28 % for 90 days and then male and female rats mated for 1∶1 pattern. The offspring of F0 was F1. F1 rats were fed to exogenous nucleotides for 90 days after they were weaned. And then these rants go on to the second reproductive experiment. Repeat the progress above until F3 rats were born. In the whole period, we observed the gestational, partal, lactational period in parent rats and the lactational period and the indexes of physiological and neural development in newborn rats.ResultsNo abnormal situation was found among the gestational, partal, lactational period in parent rats and the lactational period and the indexes of physiological and neural development in newborn rats.ConclusionAt the dose of the experiment, supplementing exogenous nucleotides was safe to reproductive function and embryonic, fetal, lactational period in multi-generation rats. 　　【Key words】 Exogenous nucleotides; Development; Safety 　　近年来国内外大量研究资料日趋表明，外源核苷酸在特定生理条件下是不可缺少的营养成分，并视个体的生长发育阶段和特定的生理条件不同而有差异[1]。当动物受到免疫挑战、肝损伤、应激、饥饿及快速生长的情况下，外源核苷酸能进入各种组织中并被吸收利用，将会节省机体从头或者补救合成的消耗从而可以优化组织功能。因此核苷酸被称为是一种“半必需”营养素或“条件”营养素。 　　目前核苷酸添加剂广泛应用于婴幼儿配方奶粉中，添加了核苷酸的配方奶粉可以改善肠道菌群[2，3]，降低腹泻的发生率[4，5]，还可以激活肠道免疫,提高其天然杀伤细胞活性,从而增强对细菌感染抵抗力[