妇科止血消痛颗粒对药物流产大鼠模型雌孕激素及其受体的影响_临床医学论文.docVIP

妇科止血消痛颗粒对药物流产大鼠模型雌孕激素及其受体的影响_临床医学论文 妇科止血消痛颗粒对药物流产大鼠模型雌孕激素及其受体的影响_临床医学论文 作者：尤昭玲,曾晶,游 卉,查亚萍,邓海燕,李丹 【摘要】 目的 观察妇科止血消痛颗粒对药物流产后大鼠血清雌激素、孕激素含量及子宫组织中雌激素受体(ER)、孕激素受体(PR)表达的影响,探讨其治疗药物流产后子宫复旧不全的机制。方法 选用SD雌性大鼠72只,随机分为空白组、模型组、产妇康组及妇科止血消痛颗粒高、中、低剂量组。建立大鼠药物流产后子宫复旧不全病理模型。采用放射免疫法检测大鼠血清中雌二醇(E2)、孕酮(P)含量,免疫组化法检测各组大鼠子宫组织中ER、PR的表达水平。结果 模型组大鼠血清中E2、P含量及子宫组织中ER、PR表达均低于空白组。妇科止血消痛颗粒高、中、低剂量组用药后,血清中E2含量升高,P含量降低,差异有统计学意义(0.05,0.01),与产妇康组比较,差异无统计学意义(Pgt;0.05)。妇科止血消痛颗粒中、低剂量组可升高ER的表达,妇科止血消痛中剂量组可升高PR的表达(0.05, 0.01)。结论 大鼠药物流产后子宫复旧不全模型E2、P、ER、PR均降低,可能影响了产后子宫内膜的修复,妇科止血消痛颗粒通过升高E2、ER、PR水平,降低P水平,发挥治疗药物流产后子宫复旧不全的作用。 【关键词】 药物流产;子宫复旧不全;妇科止血消痛颗粒;雌二醇;孕酮;雌激素受体;孕激素受体;大鼠 　　Abstract：Objective To explore the mechanism of Fuke Zhixuexiaotong Keli in rats model of medical abortion, by observing the content of E2, P in serum and the expression of ER, PR in uterine tissues. Methods Seventy-two SD female rats were randomly divided into 6 groups：blank group, model group, Chanfukang group, Fuke Zhixuexiaotong Keli high-, middle- and low-dose group. The model of medical abortion was established. The content of E2, P in serum were detected by the method of immunoradiometric assay (IRMA) and the expression of ER, PR in uterine tissues by the mothod of immunohistochemicalstaining (IHC). Results Both the content of E2, P in serum and the expression of ER, PR in uterine tissues of the model group were lower than the blank group. The content of E2 raised and P reduced in Fuke Zhixuexiaotong Keli high-, middle-, low-dose group with significant difference (0.05, 0.01), and no statistical meaning with Chanfukang group (Pgt;0.05). The expression of ER was raised in Fuke Zhixuexiaotong Keli middle- and low-dose group, and the expression of PR was raised in Fuke Zhixuexiaotong Keli middle-dose group (0.05, 0.01). Conclusion The content of E2, P, ER and PR was reduced in rats medical abortion and postpartum uterine redintegration incomplete model, that may affect the repair of postpartum uterine endometrial. Fuke Zhixuexiaotong Keli pla