头孢地嗪对免疫性肝损伤小鼠外周血T细胞亚群的影响_临床医学论文.docVIP

头孢地嗪对免疫性肝损伤小鼠外周血T细胞亚群的影响_临床医学论文 头孢地嗪对免疫性肝损伤小鼠外周血T细胞亚群的影响_临床医学论文 【摘要】 目的观察头孢地嗪对免疫性肝损伤小鼠外周血T细胞亚群的调节作用。方法 采用卡介苗联合脂多糖诱导法建立免疫性肝损伤的小鼠模型；将造模成功的免疫性肝损伤小鼠随机分为胸腺肽组、头孢地嗪大、中、小剂量组、头孢曲松组和生理盐水组，连续给药7 d，同时以正常小鼠作为对照组；采用免疫荧光染色技术，经流式细胞仪对小鼠的外周血T细胞亚群进行检测。结果 免疫性肝损伤合并败血症小鼠的CD3+(%)、CD4+(%)和CD8+(%)均明显低于正常小鼠，而头孢地嗪可有效地提高免疫性肝损伤合并败血症小鼠的CD3+(%)、CD4+(%)和CD4+/CD8+。结论 头孢地嗪可通过调整CD4+和CD8+之间的平衡，有效地提高机体的免疫功能。 【关键词】 头孢地嗪；免疫性肝损伤；T细胞亚群 　　ABSTRACT: Objective To study the regulatory effect of cefodizime on the T-lymphocyte subsets of peripheral blood in mice with immunological liver injury. Methods The mouse model of immunological liver injury was induced by Bacillus Calmette Guerin and Lipoposaccharide. The study was conducted by using completely random design. The mice with immunological liver injury were divided into thymosin group, cefodizime high-, medium-, low-dose groups, ceftriaxone group and the normal saline group. The six groups were continuously administered agents respectively for 7 days, and T-lymphocyte subsets of peripheral blood in mice were determined and contrasted with those of the normal mice treated with normal saline on the 7th day. Flow cytomytry was used to determine the effects of cefodizime on T-lymphocyte subsets of peripheral blood in mice by using immuno-fluorescence technique. Results The immunological liver injury mice were deficient because their CD3+(%), CD4+(%), CD8+(%) and the ratio of CD4+/CD8+ were lower than those of the normal mice. Cefodizime effectively increased CD3+(%), CD4+(%) and the ratio of CD4+/CD8+ of the mice with immunological liver injury. Conclusion Cefodizime effectively improves the immune function of the host by regulating the balance between CD4+ cell and CD8+ cell. 　　KEY WORDS: cefodizime; immunological liver injury; T-lymphocyte subset 　　宿主的免疫反应在病毒性肝炎的肝脏损伤中发挥着重要的作用［1-2］。肝炎患者的免疫状态与病情密切相关。在发生重型肝炎时，由于机体抵抗力低下，肠黏膜通透性增加等因素易导致细菌移位，发生感染［3-4］。因此，感染是重型肝炎患者的重要合并症，而败血症是重型肝炎患者死亡的一个重要原因［5］。 　　头孢地嗪(cefodizime, CDZM)是广谱高效的新型第3代头孢菌素。研究发现，CDZM具有明显的免疫调节功能，这是其他抗生素所不