对苯二胺分子印迹聚合物的分子识别特性_临床医学论文.docVIP

对苯二胺分子印迹聚合物的分子识别特性_临床医学论文 对苯二胺分子印迹聚合物的分子识别特性_临床医学论文 作者：张铁莉 孟祥军 贾俊芳 韩利玲 【摘要】 以对苯二胺（p-PD）为模板分子，分别以甲基丙烯酸（MAA）和丙烯酰胺（AA）为功能单体，制备了p-PD的印迹聚合物P(MAA)和P(AA)，采用色谱法考察了其分子识别特性。结果表明，P(AA)对p-PD无明显的印迹效应；而甲醇为流动相时，P(MAA)能够选择性结合p-PD分子（k′=3.57），对p-PD有显著的印迹效应（印迹因子IF=2.95），P(MAA)柱可以实现p-PD与邻苯二胺（o-PD）和对氨基苯甲酸（p-ABA）的色谱分离。通过光谱实验及HF/6-31G*量化理论计算方法，对比研究了p-PD与MAA和AA之间的相互作用。MAA与p-PD能够形成更稳定的复合物，P(MAA)对p-PD具有更好的分子识别能力。研究表明紫外吸收光谱法和荧光光谱法以及量子化学理论计算法可作为功能单体筛选的有效手段；对于荧光模板分子，荧光光谱法具有简便、灵敏等特点。 【关键词】 对苯二胺；分子印迹聚合物；分子识别；分子光谱法；量子化学计算法 Abstract Two p-phenylenediamine(p-PD)-imprinted polymers，P(MAA) and P(AA)，were synthesized using methacrylic acid (MAA) and acrylamide(AA) as functional monomer，respectively，in order to prepare molecular recognition material with high selectivity for p-PD and explore the feasibility of methods such as molecular spectrometry and computational approach of quantum chemistry for the selection of functional monomer with high imprinting efficiency.The molecular recognition properties of the imprinted polymers were evaluated by high performance liquid chromatography.The results indicated that P(AA) exhibited no imprinting effect for p-PD，while P(MAA) can bind p-PD selectively(k′=3.57)，which showed remarkable imprinting effect (IF=2.95)，and p-PD and its analogues o-phenylenediamine and p-aminobenzoic acid can almost realize baseline separation on P(MAA) column in the mobile phase of methanol.Furthermore，we made a comparative study on the interaction of p-PD with MAA and AA by spectroscopic techniques such as UV and fluorometry as well as HF/6-31G* computational approach.The results demonstrated that the complex of p-PD-MAA was more stable than that of p-PD-AA，which can give a good explanation for the molecular recognition properties of P(MAA) and P(AA).The study indicated that both molecular spectrometry（UV and fluorometry）and computational approach of quantum chemistry can be employed as efficient means for the selection of efficient functional monomer.The results showed that fluoro