慢性阻塞性肺疾病大鼠模型气虚血瘀痰阻证评价_临床医学论文.docVIP

慢性阻塞性肺疾病大鼠模型气虚血瘀痰阻证评价_临床医学论文 慢性阻塞性肺疾病大鼠模型气虚血瘀痰阻证评价_临床医学论文 【摘要】 目的 评价慢性阻塞性肺疾病(COPD)大鼠模型的中医证候特征。方法 采用气道内灌注内毒素加香烟烟熏的复合方法建立大鼠COPD模型,并对该模型从宏观状态、病理形态结构和肺功能等方面进行观测,评价该模型的中医证候特点。结果 模型组大鼠皮毛失去光泽、黄涩,爪甲紫黯,精神萎靡,汗多,活动减少,少反抗,少厮咬,呼吸深大,活动加剧,体重较健康对照组减轻,气道分泌物增多；肺组织体积增大、出现瘀血,小气道壁、平滑肌、胶原较正常对照组增厚,差异有统计学意义(P＜0.05),与正常组比较,肺呼气总阻力(Re)、最大通气量(MVV)、第0.3秒用力呼气容积与用力呼气容积的百分比(FEV0.3/FVC)、用力呼气流速(FEF25～75)均降低,差异有统计学意义(P＜0.05)。给予益气活血化痰法中药复方干预后,COPD模型大鼠上述体现气虚血瘀证的表象轻,小气道气道壁、胶原厚度均较模型组薄,肺功能Re、FEV0.3、FEV0.3/FVC、MVV、FEF25～75优于模型组,差异有统计学意义。结论 气道内灌注内毒素加香烟烟熏的复合方法建立的COPD大鼠模型符合人类COPD的病理及肺功能改变特点,同时也体现了气虚血瘀痰阻证证候学特征。 【关键词】 慢性阻塞性肺疾病；动物模型；气虚血瘀痰阻证；气道重塑 Abstract：Objective To estimate TCM syndrome of rat model with COPD. Method The rat model with COPD was made by infusing the airway with endotoxin and fumed by cigarets. The macrography, pathological morphosis and lung function of the model were observed, and the features of their TCM syndrome were estimated. Result Model rats had these features：fur tarnished, yellow and unsmooth, blue nails, spirit depressed, activity decreased, less resistance and less bite, breathe deeply and increased after activity, lose weight campared with the control group, secretion increased in the airway. The volume of lung tissue increased with stagnated blood, the wall, smooth muscle and collogen of small airway thicken. Compared with the control group, the expiratory resistance (Re), maximal ventilatory volume (MVV), forced expiratory volume in 0.3 second/forced vital capacity (FEV0.3/FVC), forced expiratory flow between 25% and 35% of forced vital capatity (FEF25～75) of model all got degraded, the difference between the two groups was significant (P＜0.05). After the intervention of TCM prescription of enriching qi, promoting circulation and dissipating phlegm, the macrography mentioned above got better, the wall and collogen of small airway thinned, the lung function such as Re, FEV0.3/FVC, MVV and FEF25～75 were better than the model, with significant difference. Conclusion The rat model