修改的茶碱壳聚糖微球对哮喘小鼠作用11.13.docVIP

茶碱壳聚糖缓释微球吸入对小鼠哮喘模型 气道炎症及气道痉挛的影响 作者单位：260041 1 潍坊医学院09级研究生 2 潍坊哮喘病医院 3 潍坊医学院附属医院检验科 4 潍坊医学院药学系 5 潍坊市第二人民医院病理科 通讯作者：温明春 wenmingchun@126.com张维芬 Email：zwf212 @126.com. 基金项目：山东省自然科学基金（编号Y2008F15）Anti-inflammatory and bronchodilator effects of theophylline/chitosan microspheres on allergic asthma of mice. ZHANG Shou-qiang, WEI Chun-hua, GAO Kun-shan, LI Zhi-jian, HOU Wen-xia, ZHU Wei-na, WEN Ming-chun, ZHANG Wei-fen. Corresponding author: WEN Ming-chun ,Department of resporatory, weifang asthma hospital. Eamil: wenmingchun@126.com. ZHANG Wei-fen. WeiFang Medical Collage. Emai: zwf212 @126.com. 【ABSTRACT】Objective : To investigate the effects of theophylline/chitosan (TH/CTS) microspheres on the the number of leucocytes and cytokines in the bronchoalveolar lavage fluid (BALF) and lung tissues in ovalbumin(OVA) sensitized and challenged mice. Method: Forty BALB/C mice were divided into four groups: including the control group , asthma model group , budesonide treatment group , TH/CTS microspheres treatment group. Mice were sensitized on days o ,7 and 14 by OVA and challenged from days 21 to 27 by OVA repeatedly to establish a asthma model .To assess the effects of BUD and TH/CTS microspheres, anminal were treated with BUD and TH/CTS microspheres .The total and differential number of leucocytes of the Bronchoalveolar lavage fluid ( BALF ) were counted by a blood analyser. Tumor neucrosis factor (TNF-α)，interleukim 4(IL-4) levels of the BALF were measured by ELISA. Lung tissues slices were stained by HE ，The lumen diameter of bronchial( T ), the area of wall ( WAt ), the perimeter of basement membrance of bronchioles (Pi) were measured by Image processing software. Results: In repeatedly OVA-challenged mice , the total and differential leucocytes counts, IL-4 and TNF-α levels increased greatly when compared with control group. Both budesonide and TH/CTS microsphers can decrease these index of inflammation. The mice treated by