PLFA分析方法在窖泥细菌鉴定中的应用初探.pdfVIP

2014年营养与食品科技学术年会“营养·食品·安全·健康”成都论坛论文(摘要)集 PLFA分析方法在窖泥细菌鉴定中的应用初探 刘燕梅；黄治国’；卫春会；邓杰；王艳丽；万世旅 (四川理工学院，酿酒生物技术及应用四川省重点实验室，四川自贡643000) 摘要本实验从浓香型白酒窖泥中筛选出两株细菌，对其进行茵落观察及生理生化检 测，参照《伯杰细菌鉴定手册》初步鉴定为芽孢杆菌属。经磷脂脂肪酸鉴定后其结果是：L1 sDNA 号菌为地衣芽孢杆菌，相似度0．916；L2号茵为巨大芽孢杆茵，相似度0．889。采用16 测序对PLFA测定结果进行验证，两株菌的结果与前面一致，相似度达97％。因此，PLFA分 析方法用于窖泥细菌的鉴定是可靠的。 关键字窖泥；细菌；PLFA鉴定 TheFirst ofPLFA in Techniques Exploration Analysis TheIdentificationofBacteriainPitMud Yanmei，Wei Shinv HuangZhiguo，Liu Chunhui，Dengjie，WangYanli，Wang (Sichuan of UniversityScience＆Engineering．LiquorMaking of ofSichuan Laboratory Province，Zigong643000，China) Key from the Abstract：Inthis strainsofbaceriawerescreened mudas starting experiment，two pit identificationto colonyobservation，andphysiologyical-biochemicalaccording strain．Through manulofbacterialidentification．andidentifiedas PLFA Bergeys preliminary Bacillus．Through identificationtheresultshowedthat：L1wasBacillus of0．91 was 6；L2 lichniformis，similarity Bacillus of0．889．To theidentificationresultsofPLA16sDNA verify by megaterium，similarity resultswas of PLFA method sequencing，the consistently,similarity97％．Therefore，theanalysis fortheidentificationmudbacteriaisre