血红密孔菌对刚果红脱色的共培养体系优化地研究.pdfVIP

／血红密孔菌对刚果红脱色的共培养体系优化的研究· 赵敏幕庆峰钱程 东北林业大学生命科学学院哈尔滨150040 摘要：在东北林区的4个自然保护区中采集、纯化得到34种野生大型真菌，对其 氧化酶系进行了定性检测，从中选出6个自腐菌菌种进行染料脱色研究，发现血 红密孔菌(Pycnoporussanguineus)对4大类染料有高效广谱脱色作用。正交试验结 果表明，在限氮条件下培养5d，其对刚果红脱色率最高可达89．04％；添加微量元 素改良培养基的正交试验表明，培养5d最高脱色率可达98．01％；在此基础上添 加诱导剂，对刚果红的脱色率则显著降低，经DPS软件处理正交试验数据，发现 添加诱导剂愈创木酚是导致脱色率降低的根本原因。分别用海藻酸钠，聚乙烯醇 固定血红密孔并进行染料脱色试验，前耆对刚果红7d脱色率达92．86％，后者对 ” 刚果红7d脱色率达22．97％。 关键词：血红密孔菌；染料脱色；培养基 THEMUTI．CUI肌瓜EDANDoPTIMIZATEDSYSTEM RESESEARCHOFP】，i|C!?v；I翻PD旧【7S|s=‘利、恰形ZNEE7SToDYE DEGRADATIoN MU ZHAOMin Q ing-fengQian-Cheng Northeast 50040 ForestryUniversity,Harb玩1 E-mail：92zb@埘gh．org collected of wild were and from Abstract：Thirty—fourspechesbig fungi purified naturalforests four areasinnortheast of throughoutnature．protected China,Arange oxidasesofwhichwereexaminedin ofwhiterot were quality．Sixspecies lungi collectedtouseinthe ofdecolorization．Ae街cient andwide study high range decolorization foundinthedecolorizationoffour fungus saBg撕Beu$was Pycnoporus kindsof testindicateddecolorizationrateof red dye．Orthogonal gongoreached 89．04％in andstill conditionforfive nitrogen—limitedlight-prevent days．The decolorizationratereached98．01％whenaddedtraceelementstOculturemedium．The rate failedinfive whenweadded dyes(Oongored)deeolorizionobviously