Z-DNA 及其生物学功能.pdfVIP

第21卷 第1期 生命科学 Vol. 21, No. 1 2009年2月 Chinese Bulletin of Life Sciences Feb., 2009 文章编号 ：1004-0374(2009)01-0072-04 Z-DNA 及其生物学功能 汤雅男，杨　攀，胡成钰* （南昌大学生命科学学院，南昌 330031） 摘　要：Z-DNA 是一种处于高能状态、不稳定的DNA 分子构象。形成Z-DNA 的原因有很多：首先， 转录过程中，移动的RNA 聚合酶在模板DNA 的 5 端产生负超螺旋扭曲力，导致Z-DNA 的形成；其 次，含有d(GC) 2+ 序列的核酸分子在高浓度的NaCl、[Co(NH ) ] 盐溶液中也能够形成Z-DNA；最后，化 n 3 6 学修饰也可以使DNA 产生稳定的 Z-DNA。Z-DNA 是在体外首先发现的，但随着研究的不断深入，发 现Z-DNA 在体内也广泛存在并可能具有功能的多样性，包括参与基因表达调控、染色体断裂、基因 重组、抗病毒、病毒发生等生物学过程。 关键词：Z - D N A ；基因表达；Z - D N A 结合蛋白；干扰素系统 中图分类号：Q52 3　　文献标识码：A Z-DNA and its biological function TANG Ya-nan, YANG Pan, HU Cheng-yu * (College of Life Science, Nanchang University, Nanchang 330031, China) Abstract: Z-DNA is an instable and high energy DNA conformation in vivo and it can be formed in many conditions. Firstly, when RNA polymerase is moving on a DNA template, transcription can result in negative supercoiling behind the polymerase, thus facilitating Z-DNA conformation at permissive regions. Secondly, the Z- DNA-conformation which is formed by d(GC) sequence can also be stabled in high-salt solution of NaCl or [Co(NH) ]2+ n 3 6 and lastly, the Z-DNA can be formed by covalent modifications. Though Z-DNA was firstly discovered in vitro, recently many evidences revealed that Z-DNA was widely existed and may possessed diversity of function in vivo, including gene expression and regulation, chro