哈密黄芪化学成分预试及生物碱成分色谱分析.pdfVIP

2009 9 29 9 Chin J Vet S ci Sep. 2009 Vol. 29 No. 9 1217 * 孙莉莎, 路 浩, 赵宝玉 , 霍星华, 万学攀, 刘忠艳, 王占新 ( , 712100) : 采用植物化学成分系统预试 生物碱系统提取 和薄层色谱技术对哈密黄芪化学成分, 特别是生物碱成分 进行了薄层色谱分析结果表明, 哈密黄芪含有生物碱多糖苷类氨基酸甾体萜类油脂鞣质酚类有机酸 黄酮醌类强心甙和香豆素2 968 g 哈密黄芪经乙醇热回流提取得303. 3 g 总浸膏, 经酸化碱化, 所得碱液依次 用氯仿乙酸乙酯正丁醇萃取, 得到3 部分浸膏分别为 0. 51, 1. 72, 19. 10 g, 提取率分别为0. 017% , 0. 058% , 0. 6 % ; 说明哈密黄芪生物碱主要集中在正丁醇提取部分, 以大极性生物碱为主对各部分提取物进行薄层层析 分析, 结果显示氯仿乙酸乙酯正丁醇提取部分生物碱至少分别有 17, 13, 5 种经与标准样品对照, 证明哈密黄 芪所含的生物碱主要以苦马豆素等吲哚里西啶生物碱为主, 同时也含有少量的黄华碱和臭豆碱等喹诺里西啶生物 碱本试验还利用薄层制备技术分离得到正丁醇提取部分中的苦马豆素 : 哈密黄芪; 生物碱; 薄层层析; 苦马豆素 : S856. 9 : A : 1005- 5 5( 2009) 09- 1217-05 Preliminary chemical test of Astrag al us hamiensis S. B. Ho and alkaloids analysis * SUN L-i sha, LU Hao, ZHAO Bao-yu , HU O Xing-hua, WAN Xue-pan, LIU Zhong-yan, WANG Zhan-xin (College of Veterinary Medicine, N orthwesternA F University , Yangling , S haanx i 712100, China) Abstract: Using phytochem istry composition preliminary systematic test, alkaloids syst emat ic extract met hod and T LC to determine the chemical components of A stragal s hamiensis S. B. H o. T he results show ed that A . ha- miensis S. B. Ho cont ained alkaloids, polysaccharides, glucosides, aminoacid, steroids, t erpenoids, oils, tannin, phenol, organic acid, f lavonoids, chinones, cardiac glycosides and coumarin. 2 968 g plant materials placed in a large round botton flask w ith indust rial alcohol and heated ref lux for several hours, the ethanol collection was concentrat ed to dryness, yield con- extract um 303. 3 g, the extractum was dissolved in acid and adjusted to alkalinity, and then extrac- ted by chloroform, acetic ether and n-butanol by turns. Three extracted fractions w ere concent rated to dryness, ob