一氧化氮对库血红细胞变形性的影响特征之探讨.pdfVIP

重庆医学2009 年11月第38 卷第21期 2695 1 1 2 2 张洪为 ,李代渝 , 赵 华 , 林 江 ( 四川省泸州医学院附属医院: 1.输血科; 2. 检验科 646000) : 研究不同浓度 一氧化氮( NO) 对库血储存期内不同时间段红细胞变形性 影响特征 血样来源于 10 个健康人,在储存 不同时相分别与4 种不同浓度NO 供体! !! 硝普钠(SN ) 一起孵育, SN 浓度分别为 151050M 孵 育后测定不同时相 库血红细胞 变形指数聚集指数刚性指数等 储存期内 库血红细胞变形性和聚集性随着时间延 长而下降, 刚性指数逐渐变大当 SN 浓度为5M 时,储存 7d 之内库血红细胞变形性有明显改善; 当SN 浓度为 10M 时, 则 能明显改善储存超过7d 库血红细胞变形性高于上述浓度 NO 会损伤相应储存时段 库血红细胞变形性NO 对红细胞 聚集性没有明显影响 NO 能明显地影响库血红细胞 变形性, 对红细胞变形性 调节具有双相性作用, 并存在浓度依赖 性通过补充相应浓度 NO, 可以明显改善库血 储存质量 : 一氧化氮; 红细胞变形性;血液流变学; 库血 :R457. 1 : A : 2009) Effects of NO on stored erythrocyte deformability in vitro ZH A N G H ongw ei 1, LI D aiy u 1 , ZH A O H ua2 , et al . ( 1. D ep ar tment of B lood T r ansf usion 2. D ep ar tment of Clin ical L a bor ator y , Af f ilated H osp ital of L uz ho u M ed ical Colleg e, L uz hou, S ich uan 646000, Ch ina) Abstract: Objective To research effects of nitric oxide on red blood cell deformability during st orage of banked blood.Methods Blood samples w ere collected from ten healt hy men and incubated with four different concentrations NO donor[ nitroprusside ( SN ) ] in order to expose it during incubation t ime to four NO concentrat ions( 1M, 5M, 10M, 50M) at different storing time. After incubation, RBC deformed index, aggregat ion index and rigidity index w ere determined by blood rheometer. Results The de formability and aggregation of RBC were decreased w ith time prolong during storage of banked blood, and the rigidity index gradu ally became larger.When the SN concentrat ion was 5M or 10M ,the RBC deformability in 7d or more than 7d storage of banked blood cells was significantly imp