水稻白叶枯病菌噻枯唑抗药突变体生物学性质研究.pdfVIP

南京农业大学学报 　2001 , 24 (4) : 33～36 　 Journal of Nanjing Agricultural University 水稻白叶枯病菌噻枯唑抗药突变体生物学性质研究 沈光斌 　周明国 (南京农业大学农业部病虫监测与治理重点开放实验室 , 南京 210095) 摘要 : 引起水稻白叶枯病的 X anthomonas oryz ae 在用药剂处理过的水稻上鉴别为抗噻枯唑的突变体。致病力和胞外酶活性 测定结果表明 , 抗药性突变体与野生敏感菌株没有明显差异 , 但抗药性突变体对离体稻叶的伤害作用及引起水稻体内脂 质过氧化程度强于敏感菌株 ; 胞外产物可以降低水稻白叶枯病菌对噻枯唑的敏感性 , 病菌产生抗药性与胞外产物关系密 切 ; 抗药突变体在无药平板上转移 10 代后 , 抗药水平明显下降 , 但致病力变化在不同的突变体间表现出较大差异 , 有 的保持较强的致病力 , 有的则完全失去致病力。 关键词 : 水稻白叶枯病菌 ; 噻枯唑 ; 抗药突变体 ; 生物学性质 中图分类号: ∶S4814 　　　文献标识码 : A 　　　文章编号 : (2001) Biological properties of resistant mutants of Xanthomonas oryzae to Saikuzuo Shen Guangbin and Zhou Mingguo ( Key Laboratory of Monitoring and Management of Plant Disease and Insects , Ministry of Agriculture , Nanjing Agric Univ , Nanjing 210095 , China) Abstract : There was no significant difference between the resistant mutants (R) and the sensitive isolates ( S) of X anthomonas oryz ae to Saikuzuo bactericide in pathogenicity and activities of extracellular enzymes. But the extracellular products of the mutants made more serious damage to the detached leaf pieces of rice than that of sensitives , and the level of lipid peroxidation induced by resistant mutants was higher than that by sensitive isolates. Extracellular products could decrease the sensitivity of X . oryz ae to Saikuzuo , and the resistance of X . oryz ae was closely related with its extracellular products. After 10 cycles of subculture on the bactericidefree medium , the resistance de gree decreased markedly , and th