内源性硫化氢在脂多糖引起的肺动脉高压中的作用.pdfVIP

生理学报 Acta Physiologica Sinica, April 25, 2008, 60 (2): 211-215 211 研究论文 内源性硫化氢在脂多糖引起的肺动脉高压中的作用 * 黄新莉 ，周晓红，韦鹏，张晓静，孟祥艳，羡晓辉 河北医科大学病理生理学教研室，石家庄 050017 摘 要：为观察硫化氢(hydrogen sulfide, H2 S)在脂多糖(lipopolysaccharide, LPS)引起的肺动脉高压中的作用，应用离体血管 环张力测定方法测定肺动脉反应性，采用生物化学方法测定肺动脉组织中H2 S 产出率和胱硫醚-γ- 裂解酶(cystathionine γ-lyase, CSE) 活性，定量PCR 方法测定肺动脉组织中CSE 表达水平。结果如下：(1) 与对照组相比，LPS 可显著升高肺动脉平均 压(mean pulmonary arterial pressure, mPAP) [(1.82±0.29) kPa vs (1.43±0.26) kPa, P0.01] ，降低肺动脉组织中H2 S 产出率 [(26.33±7.84) vs (42.92±8.73) pmol/g wet tissue per minute, P0.01]和ACh 诱导的肺动脉内皮依赖性舒张反应[(75.72±7.22)% vs (86.40±4.40)%, P0.01] ；(2) NaHS 可部分逆转上述变化，而PPG 加剧上述变化；(3) CSE 活性和CSE mRNA 表达的变化 与H S 产出率的变化相同。结果提示， LPS 对内皮依赖性舒张反应的抑制导致肺动脉高压的发生，此作用可能与H S 有关。 2 2 关键词：脂多糖；硫化氢；肺动脉高压 中图分类号：R6 31 .4 Role of endogenous hydrogen sulfide in pulmonary hypertension induced by lipopolysaccharide * HUANG Xin-Li , ZHOU Xiao-Hong, WEI Peng, ZHANG Xiao-Jing, MENG Xiang-Yan, XIAN Xiao-Hui Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China Abstract: The purpose of the present study was to explore the role of endogenous hydrogen sulfide (H2 S) in pulmonary arterial hypertension induced by endotoxin. Adult male Sprague-Dawley (SD) rats were randomly divided into four groups: Control group (0.5 mL/kg body weight of normal saline, i.v.), lipopolysaccharide (LPS)-treated group (5 mg/kg body weight of LPS, i.v.), LPS + NaHS (5 mg/kg body weight of LPS, i.v., and 28 μmol/kg body weight of NaHS, i.p.) and LPS + PPG group (5 mg/kg body weight of LPS, i.v., and 30 μmol/kg body weight of PPG, i.p.). Rats were anesthetized with 20% urethane (1 g/kg body weight, i.p.). A polyethylene catheter was inserted into the pulmonary artery through the right external ju