光对铜绿微囊藻挥发性成分的影响.pdfVIP

西 南 农 业 学 报 2010 23 5 1484 Southw est Ch in Journ l of Agricultur l Sciences Vo l23 No5 : 100 1- 4829( 20 10) 05- 1484- 05 邓国宾, 薛红芬, 汤晓莉, 杨明挚, 陈善娜, 陈小兰* (, 650091) : 采用同时蒸馏萃取装置提取收集, 并用气相色谱- 质谱联用仪对光照和黑暗条件下培 的铜绿微囊藻挥发性成分进行了 分离和鉴定分离并鉴定了 56种化合物, 光下 41种, 暗下 39种, 其中24 种为共有物质暗下生长较光下生长的铜绿微囊藻中, 醇类所占比例明显下降( 由光下 47. 69 %下降为暗下 5. 19 % ) , 烷类烯类苯类物质略有下降; 而酸类所占比例明显上升(光下为 0. 78 %, 暗下为 12. 93 % ) , 酮类胺类酯类所占比例也明显上升 : 铜绿微囊藻; 同时蒸馏萃取; 气相色谱- 质谱法; 挥发性成分 : Q949. 2 : A Effect of Light on Volatile Component ofM icrocy stis aerug inosa * DENG Guob in, XUE H ongfen, TANG X i oli, YANG M ingzh,i CHEN Sh nn CHEN X i ol n ( Pl nt Rese rch Institute ofL ife Scien ce C ollege, Yunn n Un iversity, Yunn n Kunm ing 650091, Ch in ) A bstract: The vo l tile compound s ofM icrocy stis aerug inosa Ku itz cultured under light nd d rk conditions w ere prep redw ith smi ult n eous distill tion nd extr ction equ ipm ent( SDE). Th ey w ere iso l ted nd identified by c p ill ry GC/MS m ethod. 56 subst n cesw ere identified to t lly. 41 of them w ere isol ted from culture s m ples grow th under light, wh ile 39 under d rk. C om p red to s mples cultured under light, the vol tile compon ent of cells from d rk culture cont inedm ore cids( 12. 93 % to 0. 78% ) , ketenes, m ic m ines nd ester, w h ile th e r tio of lcohols decre sed m rkedly from 47. 69 % to 5. 19 % , so s lkyls, olefins nd benzenes. Key words:M icrocy stis aerug inosa; Smi ult neou s distill tion nd extr ction equ ipm ent( SDE) ; GC /M S; Vol tile com pounds ,