半夏生物总碱对癫痫大鼠海马氨基酸含量和GABAA受体mRNA表达的影响.pdfVIP

蠢鼍譬 。|。2观t| 一 Jo1栅 oSChinesePharmacetlticalSciences ht~o．／／www．jcps．ac．cn Theeffectsofpinelliatotalalkaloidsontheaminoacidconcentrationand theGABAAreceptorexpressioninhippocampusregionofepilepticrats Yan-TingGu，Yong．GangMa，M ing—ZhengW ang，Xin—FengW ang，YiXiao DepartmentofPharmacology,ShanxiMedicalUniversity,Taiyuan030001，ShanxLChina Abstract：TheantiepilepticeffectofpinelliatotalalkaloidsfPTA onpenicillin(PNC)chronicallykindledratswasinvestigated． Weinvestigated也eeffectsofPTAonGlu，Asp，Glyandy-aminobutyricacid(GABA)concentrationsnadtheexpressionlevelof cerebralGABA-Areceptorni hippocampus．ThenifluenceOfPTA onepilepsyseizurelatnecyanddegreeinPNCchronicallykindled ratswereobserved．Highperformnaceliquidchromatography(HPLC)wasadoptedtomeasurehteconcentrationsofGlu，Asp， GlyandGABAinhippocampus．Reversetrnascriptase-polymerasechanireaction(RT．PCR1wasusedtodeterminetheexpression ofcerebralGABAAreceptormRNA．Comparedwiht normalrats，thelevelsofGABA nadGlydecreasedobviouslywhilehte levelofGlunadAspincreasedsignificnatlyinmodelrats．Th ecerebralGABAAreceptormRNA levelwasalsodecreasedatthe sametime．乃 edifferencewasstatisticallydifferentcompraedtothecontrolgroup．PTA couldprolongthelatentperiodofthe pneicillniniducedseizurenadweakenhteextentofseizure．compraedwiththemodelrgoupwihtoutPTAtreatment．Moreover．PTA increasedthelevelofGABA nadhteexpressionlevelofGAB AArec印tor，whiledecreasedhtelevelofGlusignificnatly．However， ithadnoobviouseffectonhtelevelofGlyandAsp．Pre．treatmentOfPTA canalsonicreasetheGABAArece口tormRNA leve1． Inconelusion．PTA couldalleviatethePNC chronicallykindledratseizure．ItincreasedhteGABA levelna dhteexpressionof GABA_Areceptor，naditdecreasedtheGluconcentration． Keywords：Pnielliatotalalkaloids TA)