猪源马链球菌兽疫亚种中国分离株类M基因特性分析.pdfVIP

中国农业科学 2006,39(1):210-214 Scientia Agricultura Sinica 猪源马链球菌兽疫亚种中国分离株类M基因特性分析 范红结，陆承平，唐家琪 （南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室，南京 210095 ） 摘要：【目的】阐明国内不同区域猪源马链球菌兽疫亚种分离株类M基因的特性，为疫苗研制提供指导。【方 法】利用PCR 技术，扩增国内9省市从1974～2003年分离的9株马链球菌兽疫亚种类M基因，并进行克隆、测序 及序列分析。【结果】发现 7 株类 M 基因长度为 1 137 bp，含一个阅读框，CG-74-63 和 CP-0104 株的长度分别为 1 143 bp及1 125 bp。除1株关节炎分离株CP-0104外，引致败血症的8株菌类M基因的核苷酸同源性高达95.4%～ 99.9%，推导的相应氨基酸序列同源性高达 92.1%～100%。9 株猪源菌与马源菌株及人源菌株类 M 基因的核苷酸同 源性分别为 81.6%～87.0%、81.7%～91.8%。9 株猪源分离株类 M 蛋白信号肽、C 末端细胞锚定区的序列均高度同 源；除CP-0104外，其余 8株高变区高度同源。【结论】国内不同区域猪源马链球菌兽疫亚种分离株类M基因同源 性高。 关键词：马链球菌兽疫亚种；类M基因；分析 Analysis of the M-Like Gene of Streptococcus zooepidemicus Chinese Isolates from Pig FAN Hong-jie , LU Cheng-ping, TANG Jia-qi （Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture ， Nanjing Agricultural University, Nanjing 210095 ） Abstract: 【Objective 】In order to clarify the features of the M-like gene of Streptococcus equi subsp. zooepidemicus isolates from Chinese pig for directing the selection of vaccine isolate. 【Method 】The M-like genes were amplified from 9 isolates of Streptococcus equi subsp. zooepidemicus from Chinese pig by PCR and the M-like gene of each isolate was cloned, sequenced and analyzed. 【Result 】Nucleotide sequence analysis revealed one open reading frame in M-like protein gene of all isolates, with different length of 1 125 bp in CP-0104 isolate, 1 143 bp in CG-74-63 isolate, and 1 137 bp in the others. The nucleotide identities of M-like genes among 9 isolates except CP-0104 isolate were higher than 95.4%, while the identities between isolate CP-0104 and the remaining 8 strains were 87.1%-88.