柿胡萝卜素脱氢酶基因的克隆与序列分析.pdfVIP

生物技术通报 # # BIO TE CHN OLOG Y B ULLETIN 20 11 2 F- 赵大球 周春华 薛银芳 陶俊 (, 225009) : 通过对柿 F-胡萝卜素脱氢酶 ( F-Carotene desaturase, ZDS)基因的克隆及序列分 , 旨在为改良柿果实品质奠定 基础根据 GenBank 中其他植物 ZDS基因的保守序列设计了两条 PCR 扩增引物, 以柿果实中所提取的 RNA 为模板, 采用 RACE技术扩增出一条约为 500 p的条带经过序列分 , 其长 578 p, 不含内含子, 具有部分开放阅读框 ( 177 p)终止密 码子( TGA) 和 poly(A)尾巴( 11 p), 共编码 58 个氨基酸该序列及其所编码的氨基酸与其他植物 F-胡萝卜素脱氢酶基因均 具有较高的同源性该基因已提交 GenBank数据库, 登录号为 GU075728 : 柿 类胡萝卜素 F-胡萝卜素脱氢酶基因 克隆 序列分 Cloning and SequenceAnalysis of F-CaroteneDesaturase Gene in Persimmon(D iospy ros kak i L1) Zhao Daqiu Zhou Chunhua XueY infang T ao Jun (College of H orticulture and P lantProtection, Yangzhou University, Yangzhou 225009) Abstrac:t To lay the foundation for mi proving the quality of pers mi m on fru it, F-carotene desatu rase( ZDS) gene w as cloned from persmi m on. Tw o PCR p rmi ersw ere des igned accord ing to conservative sequence of ZDS gene in other p lants reported in G en- Bank. TotalRNA of persmi m on fruit as tem plate, a 500 p fragm entw as am p lified according to RACE techno logy. Sequence analy- sis show ed that the fragm en t w as 578 p and en coded 58 am ino acids. H om ology analysis show ed that oth nucleotide and am ino acids sequence shared h igh smi ilaritiesw ith ZDS genes cloned from other plants. Th is gene had een su m itted to G enB ank data- ase w ith GU075728. K ey w ords: Persmi mon(D iospyros kaki L. ) Carotenoid F-C arotene desaturase gene C lon ing Sequence analysis [ 7] [ 8] [ 9] (D iospyros kaki L1), , [ 1, 2] [ 10] , ,