降钙素原在新生儿感染性疾病早期诊断中的应用.docVIP

降钙素原在新生儿感染性疾病早期诊断中的应用 　　作者：朱玲玲，舒桂华，严语，徐翔(江苏省苏北人民医院新生儿科 江苏扬州 225001) 　　Expression of CTGF in pulmonary vessels of hypoxemic pulmonary hypertensive mouse 　　ZHANG Qiang，LIN Yong 　　(Department of Respiratory，Zhongda Hospital，Southeast University，Nanjing210009，China) 　　Abstract:Objective To establish a model of hypoxemic pulmonary hypertensive mouse and to explore the expression of connective tissue growth factor(CTGF)in the pulmonary arteriole from pulmonary hypertensive mouse.Methods The model of hypoxemic pulmonary hypertensive mouse was constructed by a method of interval ordinary pressure hypoxia.Right cardia catheterization was employed for mPAP detection，and RV.(LV+S)was determined by weight.Lung tissue section staining and image processing were performed to detect the morphologic change of pulmonary arteriole.CTGF levels in the pulmonary arteriole were determined by immunohistochemistry，and the concentration was measured by image pattern analysis and semi-quantitative technique.Results Three weeks later，the results of mPAP，RV.(LV+S)，and the levels of WT%and WA%indicating the proliferation of vessel wall in the group of hypoxemic group were higher than those in normal group(P0.01).There was no expression of CTGF protein in the pulmonary arteriole of the normal group，whereas，it was highly expressed in the tunica adventitia，slightly expressed in the endangium and rarely expressed in the tunica media of pulmonary arteriole in the hypoxemic group.Conclusion Method of interval ordinary pressure hypoxia can successfully construct hypoxemic pulmo-nary hypertensive mouse model.CTGF may play an important role in hypoxia pulmonary vascular remodeling. 　　Key words:hypoxia;pulmonary vascular remodeling;connective tissue growth factor 　　[摘要]目的: 探讨新生儿感染性疾病早期诊断的方法和降钙素原(PCT)在新生儿感染性疾病诊断中的临床意义。 方法: 对76例疑似新生儿感染患儿，在应用抗生素前测定PCT、C反应蛋白(CRP)、血常规及行血培养，比较它们对细菌感染诊断的敏感性、特异性。 结果: (1)血清PCT检测对细菌感染诊断的敏感性为81.36%，特异性为64.71%。(2)CRP对细菌感染诊断敏感性为47.46%，特异性为70.59%;与血清PCT检测结果比较，敏