本科毕业论文-吸烟对人体可替宁、MDA和8-OHdG水平的影响.docVIP

吸烟对人体可替宁、MDA和8-OHdG水平的影响 指导老师 学 生 郑州大学公共卫生学院教研室 郑州 45000 【摘 要】:目的：探讨吸烟对人体可替宁、MDA和8-OHdG水平的影响。方法:以被调查的“某自然村的男性村民”为源人群收集114例健康研究对象的血样，根据日吸烟量（0支/天即不吸烟对照组，≤20支/天和＞20支/天共3组）与烟龄（0年即不吸烟对照组，≤26年，≤33年和＞33年共4组）分组。检测血浆中丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）和血清中可替宁的浓度水平，以反映吸烟引起的机体氧化损伤水平。结果:按吸烟量分组，与“0支/天不吸烟对照组”相比，“≤20支/天”和“＞20支/天”两组的可替宁、MDA、8-OHdG含量显著增加，差异有统计学意义（P0.05），且“≤20支/天组”指标含量小于“＞20支/天组”，差异有统计学意义（P0.05）；按烟龄分组，与“0年不吸烟对照组”相比，≤26年，≤33年和＞33年三组的可替宁、MDA、8-OHdG含量显著增加，差异有统计学意义（P0.05），且以＞33年组的含量最高，差异有统计学意义（P0.05）。结论:随着吸烟量与吸烟时间的累积，人体内可替宁、MDA和8-OHdG浓度有升高趋势，对人体有害效应的危险性不断增大。因此，戒烟或尽可能少吸烟，养成良好的生活习惯，有助于消除和减轻烟草中有害物对机体的危害。 【关键词】:丙二醛（MDA），8-羟基鸟苷（8-OHdG），可替宁，氧化损伤。 The influences of smoking on cotinine，MDA and 8-OHdG levels of Tutor Zhang Qiao Student Wang Liwei Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001，China Abstract：ObjectiveTo explore the influences of smoking on cotinine，MDA and 8-OHdG levels of. Methods：Collect blood samples from 114 heathy research objects from a village and divide them into several groups according to smoking count every day(3 groups:0 No. of cigarette /day, ≤20 No. of cigarette /day and ＞20 No. of cigarette /day ) and smoking duration(4 group:0 year, ≤26 years, ≤33 years and ＞33 years). Then detect the concentration of MDA,8-OHdG and cotininen order to reflect the level of oxidative damage caused by smoking.Results:Grouped by smoking count every day,compared with control group, ANOVA analysis of the MDA, 8-OHdG in plasma and Cotinine concentration in serum of the other two groups showed that there was statistically significance among them (P0.05);the contents of MDA, 8-OHdG and Cotinine in the ＞20 No. of cigarette /day group were much higher than those in the ≤20 No. of cigarette /day group,and there was statistically significance among them(P0.05);Grouped by smoking duration,compared with control group, ANOVA analysis of the MDA, 8-OHdG in plasma and Cotinine concentration