几种抗生素与人血清白蛋白结合反应的研究_毕淑云.pdfVIP

2005 63 Vol. 63, 2005 23 , ACTA CHIMICA SINICA No. 23, · · a,b a a a *,a (a 130012) (b 133002) , 26 K 4 3 3 4 4 1 A 1.9810 , 1.01 10 , 1.38 10 , 5.97 10 7.15 10 L•mol , n 1.16, 0.86, 1.19, 0.91 0.93. . ; ; ; ; ; ; Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin BI, Shu-Yuna,b DING, Lana SONG, Da-Qiana TIAN, Yuana ZHANG, Han-Qi*,a a ( College of Chemistry, Jilin University, Changchun 130012) b ( Chemistry Department, College of Science and Engineering, Yanbian University, Yanji 133002) Abstract The binding characteristics of antibiotics, cefotaxime sodium, oxacillin, amoxicillin, norfloxacin and enoxacin with human serum albumin (HSA) were studied by fluorescence spectrometry. These drugs quenched the HSA fluorescence via a nonradiative energy transfer mechanism. The binding constants (KA) and binding sites (n) for drug-HSA were obtained by an improved analysis method to process the experi- 4 3 3 4 4 mental data. The values of KA obtained were 1.98 10 , 1.01 10 , 1.38 10 , 5.97 10 and 7.15 10 1 L•mol for cefotaxime sodium-HSA, oxacillin-HSA, amoxicillin-HSA, norfloxacin-HSA and enox-