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Acta Physiol Plant (2011) 33:2511–2520 DOI 10.1007/s11738-011-0796-7 ORIGINAL PAPER Analysis of influences of spaceflight on chemical constituents in licorice by HPLC–ESI-MS/MS Jing-Ze Zhang • Wen-Yuan Gao • Ying Gao • Dai-Lin Liu • Lu-Qi Huang Received: 17 February 2011 / Revised: 1 May 2011 / Accepted: 30 May 2011 / Published online: 14 June 2011 ´ ´ Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, Krakow 2011 Abstract Focusing on the variations of chemical con- flavonoids, 16 triterpene saponins and 1 coumarin were stituents in licorice root, influences of exposure to physical identified according to their mass spectra determined in factors of spaceflight on licorice (Glycyrrhiza uralensis both negative and positive ion modes. The research pro- Fisch.) seeds were investigated. Licorice seeds obtained vided the scientific data for spaceflight breeding of from two different producing areas were flown on a medicinal plant and indicated that the technology of recoverable satellite for 18 days. After returning to earth, spaceflight may be a new effective method for the breeding the seeds carried by the satellite and the parallel ground and cultivation of licorice. control were cultivated to maturity under the same condi- tion. Chromatographic fingerprint of 1 year licorice root Keywords Spaceflight Licorice Chemical analyzed by high performance liquid chromatography with constituents HPLC–ESI-MS/MS diode-array detection not only displayed the contents of glycyrrhizic acid and liquiritin increasing in the spaceflight samples but showed the variation of the kinds of chemical Introduction constitu

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