共轭双键对脂肪酸紫外吸收影响.pdfVIP

共轭双键对脂肪酸紫外吸收的影响 刘凤霞19薛刚1，王莹1，冯建波2 (1．南阳理工学院生物与化学工程学院，河南南阳473004； 2．河南南阳普康药业有限公司，河南南阳473004) [摘要]目的，测定共轭亚油酸及部分非共轭脂肪酸的紫井吸收，研究共轭双键对脂肪酸紫鲆吸收的 影响．方法t测定多种脂肪酸及其混合物的吸光度．井利用气相色谱法测定脂肪酸组分含量．结果：当加入 少量的共轭亚油酸时．混合脂肪酸的紫外吸收曲线表现出明显变化．结论：共轭双键对脂肪酸在230nm^’240nm 范围内的紫外吸收有显著影响，从而为共轭成分的存在提供了检测依据。 [关键词]紫外分光光度法；气相色谱法；亚油酸；共轭亚油酸；Y一亚麻酸 Influence doublebondOiltheultraviolet acids offatty ofconjugated absorption (1．Department Engineering,NanyangInstituteofTechnology，Nanyang ofBiochemical 473004,Ch／ha, Pharmaceutical 473004．China) 2．NanyangPukang Co．Ltd．，Nanyang mea,mretheultraviolet valueof linolicneidandsome [Abstnd]Objective：Toabsorption conjugated influenceof doublebond∞the of ultraviolmabsorlnion unconjugatedfattyacids，tom由tim conjugated fatty acids．Method．Tomc8aImtlm valueofsome acids Ultraviolet ultravioletabsoq口60n fatty andtheir by complex tomeawJretlm with method．P．emlt：When Spectrophotometry．Andfatty矗cid$formationgaschromatographic linolicwas ere Onthe acids’ultraviolet slight acid conjugated added。theresignificancechanges fatty complex ialfvewithinthe from to value acids 230hm240hm．Conclusion：Theultraviolet ebsorlnion lange absorptionoffatry is withinthe from230hmto240hmduetotheexistenceof double verySignificance range conjugated