The experiment study on LMWH inhibiting the expression of Livin and inducing the apoptosis of osteosarcoma cells.pdfVIP
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The experiment study on LMWH inhibiting the expression of Livin and inducing the apoptosis of osteosarcoma cells.pdf
维普资讯
TheexperimentstudyonLMW H inhibitingthe
expressionofLivinandinducingthea
ofosteosarcomacells
LeiYi,FengjinGuo,AnminChen
DepartmentofOnhopedics,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,
…
han430030.China
Received:6June2007/Revised:28July2007/Accepted:12August2007
Abstract Objective:ToinvestigatetheinhibitoryeffectsofLMWHsuppressingtheexpressionofLivinandinducingthe
apoptosisoflheosteosarcomacells.Jwe纳ods Osteosarcomacellsi『neMG一63wasculturedn『vitro
. MiTassayandflow
c~ometrywereusedlostudylheeffeclofLMWHwithdifferenlconcentrationsuppressedlheprolifetationandinducedapop.
1osisinosteosarcomacellslineMG一63.TheexpressionofLivinofosteosarcomacellslineMG.63wasanalysedbylheim.
munohistochemistricaImethodandPT-PCR.Results:Lowmolecularweightheparincouldinhibitthegrowthofosteosarcoma
ceI『IineMG一63.WiththeLMWHsincreasing,theapoptosisratewasincreasedsignificantly
. Immunohistochemistriaclmethod
andPT-PCRshowedthattheexpressionofLivinofosteosarcomacellslineMG.83declinedobviouslythanthatbeforemedi.
cation.Conclusion:LMWHhasvery stronganti—tumoreffeclinvitro. ThepossiblemechanismsofLMWHanti.tumoreffecI
areassociatewiththeeffectofsuppressingtheexpressionofLivinandinducingcellapoptosis
.
Keywords LMWH;osteosarcomacell;Livin;apoptosis
Heparinisaclassictypeofanticoagulantsubstances, Louis,USA).Theparenteralsolutionoflow molecular
suchasLi’sreports_1】.Heparincanincreasethea
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