5-羟色胺转载体抑制剂对5-羟色胺作用下肉鸡肺动脉平滑肌细胞增殖和凋亡的影响.pdfVIP

禽病专题 囝 1153 T030060 5．羟色胺转载体抑制剂对5．羟色胺作用下肉鸡肺动脉平滑肌 细胞增殖与凋亡的影响 潘家强·，韩前彪，曾健滢，李英，吴玄光，唐兆新 (华南农业大学兽医学院，广东，广州，510642) 摘要t通过lift比色法研究不同浓度的5一羟色胺转|jL体抑制剂氛西汀对内坞PASMC的毒性作用．结果显示． 少于氛西汀单独处理姐．研究表明5一羟色胺转|jL体在调节内鸡肺动脉平滑肌细胞的增殖和凋亡方面起重要作用． 关t词。内鸡肺动脉平滑肌细胞，5一羟色胺，5一羟色胺转|jL体，氛西汀 Effectofserotonin inhibitoroncell andcell transporter proliferation invitroculturedbroiler smooth of musclecells apoptosis pulmonaryartery undertheactionofserotonin PAN Qian-biao。ZENG Zhao-xin。 Jia-qiang。HAN Jian-yin，LIYing。WUXuan-guang，TANG of China VeterinaryMedicine，South 0642) (Co,lege Agricultural of Abstract：Thetoxiceffectsofdifferentconcentrationsfluoxetineonbroiler smooth pulmonaryartery musclecell(PASMC)werestudiedbvMTTcolorimetric resultsshowedthat10。mol／L0f assay．The fluoxetineC811 inhibitthe ofPASMC．Therfledianinhibitionconcentrationof significantly proliferation fluoxetinetObroilerPASMCis effectsoffluoxetineoncell andcell 84．2lxmol／L．1me prolifcrationapoptOsis PASMCundertheactionserotoninwerestudied MIT and ofbroiler using assay，flowcytometry Hoechst33258fluorescencemethord．TheresultshowedthatfluoxetineCaninhibitthe staining proliferation effectofserotonintoPASMCinvitro．5X101mol／Loffluoxetine