Protein Expression amp; Purification.docVIP

Protein Expression Purification Custom Service Requirements Form Please submit completed form to geneartprotein@. Contact Information: Name: Organization: Address: Email: Phone Number: Nature of Inquiry: Pricing estimate Quote for ordering For Grant Submission Estimated Order Initiation: 1-2 Months 2-4 Months 4 Months Other: General Information: Service(s)desired: Expression Purification Other: Intended Use: Research use only Clinical applications Note: For this service area, Invitrogen produces materials for research use only. We can perform development work related to future clinical applications, but are not GMP-certified. Target: Protein Name: Accession #: Protein Sequence: Target Properties: Species: MW: Biosaftey Level: Expression System desired: Mammalian Baculovirus Target Expression: Localization: Cytosolic Secreted Nuclear Periplasm Other: Solubility: Soluble Insoluble Unknown Estimated Yield (if known): Comments: SECTION I Expression plasmid construction Starting Material: Gene Synthesis (Gene Optimization desired? Yes/No) Other (e.g. expression plasmid already stored at Life Technologies): Affinity Tag(s): 6xHis: N-term C-term GST: N-term C-term DYKDDDDK: N-term C-term Protease site? Yes: No V5 Tag: N-term C-term Myc Tag: N-term C-term Other: N-term C-term Comments:  SECTION II Expression Service System Desired Starting Material Small Scale Feasibility FreeStyle?293 FreeStyle?CHO Expression Vector DNA Vector Name: Other: Small scale expression and time course Baculovirus Expression Vector DNA Vector Name: Other: Small scale expression and time course Expression Scale: 1L