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SAT2生物知识下载:Protein Synthesis
小编
Now that we’ve described DNA and RNA, it’s time to take a look at the process of protein synthesis. The synthesis of proteins takes two steps: transcription and translation. Transcription takes the information encoded in DNA and encodes it into mRNA, which heads out of the cell’s nucleus and into the cytoplasm. During translation, the mRNA works with a ribosome and tRNA to synthesize proteins.
Transcription
The first step in transcription is the partial unwinding of the DNA molecule so that the portion of DNA that codes for the needed protein can be transcribed. Once the DNA molecule is unwound at the correct location, an enzyme called RNA polymerase helps line up nucleotides to create a complementary strand of mRNA. Since mRNA is a single-stranded molecule, only one of the two strands of DNA is used as a template for the new RNA strand.
The new strand of RNA is made according to the rules of base pairing:
DNA cytosine pairs with RNA guanine
DNA guanine pairs with RNA cytosine
DNA thymine pairs with RNA adenine
DNA adenine pairs with RNA uracil
For example, the mRNA complement to the DNA sequence TTGCAC is AACGUG. The SAT II Biology frequently asks about the sequence of mRNA that will be produced from a given sequence of DNA. For these questions, don’t forget that RNA uses uracil in place of thymine.
After transcription, the new RNA strand is released and the two unzipped DNA strands bind together again to form the double helix. Because the DNA template remains unchanged after transcription, it is possible to transcribe another identical molecule of RNA immediately after the first one is complete. A single gene on a DNA strand can produce enough RNA to make thousands of copies of the same protein in a very short time.
Translation
In translation, mRNA is sent to the cytoplasm, where it bonds with ribosomes, the sites of protein synthesis. Ribosomes have three important binding sites: one for mRNA and two fo
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