高浓度HBsAg定量检测中标本稀释度的研究.pdfVIP

·322· JClinTransfusLabMed，October．2012，Vol14，No．4 高浓度HBsAg定量检测中标本稀释度的研究 王萍王坤 王海涛余明杰 【摘要】 目的 度HBsAg患者的血清按照不同的稀释倍数稀释后进行测定。结果86．1％的标本稀释101倍后能检测出结果，96．7％的标 IU／ml时，建议选择300倍稀释后检测。 稀释倍数的增加，所得检测结果的数值在增加。结论当HBsAg250 【关键词】乙型肝炎病毒表面抗原 稀释倍数 化学发光技术 R446．6 【中图分类号】R512．6+2 【文献标识码】A 【文章编号】 1671—2587(2012)04一0322一03 Researchofthe forSerum Concentrationof in I矿ANG DilutabiIity ContainingHigh HBsAgHBsAg—quantitatiVeAssay K帆，wANG s Ping，WANG Hni一￡口0，et口z．DepartmentofLaboratory。Pe0酗dHos加tdlo}Bozho扎，B0zho札236800 【Abstract】 To the dilutiontimesfor of concentrationof in objectiveinvestigateappropriate sampIeshigh HBsAg determination．Methods122casesof with concentrationof weI．edilutedwiththediffer— quantitative sampleshigh HBsAg entle垤lsofserumdilutiontimes．Results the were detectedafter 101dilut— 86．1％ofspecimensuccessfuUy successfully ed the were detectedafterdiluted201 the weresuccess— times，96．7％ofspecimen times，99．2％of successfully specimens detectedafterdiIuted301 the were detectedafterdiIuted601times．These times，100．O％of successfully fuIly specimens revealedthesuccessfulrateofthe increased thedilutedtimes．ConclusionWhentheconcentrationof assay alon譬with exceeds250 shouldbedetectedafter timesofdilution． HBsAg IU／ml，it 300 DilutedtimesChemiluminescence 【Keywords】HBsAg analysistechnique 乙型肝炎病毒(HBV)属嗜肝DNA病毒科，完的测定结果，现报道如下。 整的HBV称为Dane颗粒，其包膜含有乙肝表面抗