肝癌组织中MAGE—A1表达及其临床意义.pdfVIP

维普资讯 · 332 · 临床与实验病理学杂志 JClinExpPathol2005Jun；20(3) 肝癌组织中MAGE—A1表达及其临床意义 肖 刚，张文敏，张 萌，谢 丹，郭爱林，文剑明 [摘要] 目的 检测黑色素瘤抗原一A1(MAGE—A1)在肝癌组织中表达，探讨MAGE—A1基因与肝细胞肝癌(HCC)患者临床病 理指标的关系。方法 应用RT—PCR法检测31例HCC组织及相应癌旁组织中MAGE—A1基因的表达。应用组织芯片和免疫 组化技术分析178例HCC组织及相应癌旁组织中MAGE—A1抗原表达。结果 31例HCC组织标本中有21例(67．74％)表达 MAGE—A1基因，而相应的癌旁组织MAGE—A1基因均不表达。178例 HCC组织 中95例表达 MAGE—A1抗原(53．37％)，相应 的癌旁组织MAGE—A1抗原均不表达。结论 MAGE—A1基因在 HCC组织 中有较高表达，其阳性率与HCC患者的临床病理指 标无相关关系。 [关键词] 肝肿瘤 ；MAGE—A1；逆转录一聚合酶链反应 ；组织芯片；免疫组织化学 [中图分类号] R735．7；R392．11 [文献标识码] A [文章编号] 1001—7399(2005)03—0332—03 ExpressionofMAGE-A1anditsclinicalsignficanceinhepatocellularcarcinoma XIAOGang，ZHANGWen—min，ZHANGMeng，XIEDan，GUOAi—lin，WENJian—ming (DepartmentofPathology，SUNYat—senMedwalCollegeofSUNYat—senUniversity，Guangzhou 510080，China) [Abstract] Purpose Toinvestigatetheexpressionofmelanomaantigen—A1(MAGE—A1)geneinhepatocellularcarcinomasnad studytherelationshipbetweentheexpressionofMAGE-A·1genesandtheclinicpathologicalcharacterizationsofpatientswithhepatoce1．． 1ulracracinomas(HCC)．Methods TheexpressionsofMAGE—A1genein31samplesofhepatocellulracracinomasandcorresponding adjacentnoncanceroustissuesweredetectedbyreversetranscription—polymerasechainreaction(RT—PCR)．MAGE—A1antigenwas detectedusingimmunohistochemistryin178caseshepatocellulracracinomasandcorrespondingadjacentnoncnaceroustissuesbytissue microarraytechnique．Results Inthe31hepatocellularcracinomasstudied，MAGE—A1mRNAWasdetectedin21cases(67．74％)． Noneofthecorrespondingadjacent tissuesexpressedMAGE—A1mRNA．Inthe178hepatocellulracracinomas studied， MAGE—A1antigenWaSdetectedin95cases(53．37％)．NoneofthecorrespondingadjacentnoncanceroustissuesexpressedMAGE—A1 antigen．Conclusions MAGE—A1geneisexpressedinahighpercentageofHCC，butitisunrelatedtohteclinicpahtologicalchraac— terizationsofpatientswithHCC． [Keywords] hepatocellulraneoplasms；melnaomaantigen—A1gene；reversetranscripase—polymerasechainreaction：tissuemicrora- ray；immunohistochemistry 199