中药华蟾素的镇痛实验研究.pdfVIP

安 徽 医 药 　A nhu iM ed ica l and P ha rm aceu tica l J ou rna l　20 10 Ap l; 14 (4) ·397 · 中药华蟾素的镇痛实验研究 邓李蓉 ,陈　涛 ,巩仔鹏 ,胡月琴 ,余雪琴 (三峡大学医学院 ,湖北 宜昌　443002) 摘要 : 目的　探讨华蟾素注射液的镇痛作用及其机制 。方法 　采用小鼠热板法 、小鼠醋酸扭体法观察两种浓度华蟾素注射液的 镇痛作用 ;采用盐酸纳洛酮实验研究华蟾素注射液镇痛作用机制 。结果 　与氨酚羟考酮比较 ,华蟾素注射液能使醋酸所致小鼠 ( ) ( ) ( ) 扭体次数减少 P 0. 0 1 ;并能提高热板所致小鼠舔足的痛阈 P 0. 05 。而且腹腔注射 IP 0. 04 m g纳洛酮能明显地拮抗华 蟾素的镇痛作用 (P 0. 00 1) 。结论 　华蟾素具有镇痛作用 ,其镇痛作用可能与阿片受体有一定的关系 。 关键词 :华蟾素 ;镇痛作用 ; 中药 Ana lgesic effect of the trad itiona l Ch inese m ed ic ine c inobufag in injection D EN G L irong, CHEN Tao, GON G Zip eng, HU Yueqin , YU Xueqin (M edical Co llege, Three Gorge s U n iversity, Yichang, Hubei 443002 , Ch ina ) A b stract:A im 　To inve stigate the analgesic effect and its m echan ism of cinobufagin injection. M ethods　A nalge sic effect of two differ en t concen tration s of cinobufagin injection wa s ob served in m ice by the hotp late te st and w rith ing w ith acetic acid. Moreover, the poten tial analgesic m echan ism of cinobufagin injection wa s inve stigated by injecting naloxone hydroch loride in to m ice. Results　Comp ared w ith oxycodone acetam inophen, the number of w rith ing induced by acetic acid in m ice can be sign ifican tly reduced by cinobufagin injection ( P ) ( ) 0. 0 1 . In addition, the p ain thresho ld of p awlick ing induced by the hotp late wa s boo sted in m ice P 0. 05 . A lso, the analgesic ( ) e