耐热酸性_淀粉酶产生菌的分离鉴定及酶学特性的初步研究_崔志峰.pdfVIP

2008 No.10 ·3　　　4 · SerialNo.187 ChinaBrewing　　　 Research Report α- 崔志峰, 罗文杰,吴春程,杨　霄 (浙江工业大学 生物与环境学院, 浙江 杭州310032) 　:, 16s rDNA (Bacillales bacterium.), Bacillales bacterium.SF-8。6440U/mL(60℃、pH5.0), 60℃ 100min 66%,4℃10d 57%。 2+ 2+ 2+ 2+ a , Fe ,Mg ,Mn 。 60kDa。SF-8 , 。 　　:α-;16SrDNA ; :Q93-331　　　　　　:A　　　:0254-5071(2008)10-0034-03 Isolation and characterization of a thermostable and acid-stable α-amylase producing strain ＊ UI ZhiFeng , LUO WenJie,WU hun heng,YANG Xiao (College of Biological and Environmental Engineering, Zhej iang University of Technology , Hangzhou31003 , China) Abstract:A new thermostable and acid-stable α-amylase producing strain was isolated from the soil of Hangzhou and identified as Bacillales bacterium.SF-8 by 16s rDNA.The maximum activity of α-amylase produced by SF-8 was6440 U/ml (60℃, pH5.0).This enzyme wasstable to high temperature, 66%of enzyme 2+ 2+ activity was retainedwhen disposed at 60℃for 100 min.After stored at 4℃for 10 days, it still has 57%of activity.The presence or absence of a , Fe , 2+ 2+ Mg and Mn did not affect its enzyme activity and thermal stability.The enzyme activity stain experiment indicated the enzyme wasapproximately 60kDa.It is meaningful to further study the characterizations of this stain for the application in industry. Key words:Thermostable and acid-stableα-amylase;16S rDNA identification;Bacillales bacterium α-,α-1, 4--4- (α-1, 4-glucan-4-glucanohydrolase E .3121111), a-SF-8,16s rDNA 、、、、, (B acillales bacterium ), ,