尖吻蝮蛇凝血酶的止血作用和其作用机制的研究_吕慧敏.pdfVIP

中国实验血液学杂志 Jou r al of Expermi e tal H em ato logy 2008; 16( 4): 883- 885 # 883# (Article ID ): 1009- 2137(2008)04- 0883- 03 # # 1 吕慧敏, 李长龄 , 董金婵, 张翠兰, 周湘艳 中国疾病预防控制中心辐射防护与核安全医学研究所, 北京 100080; 1 北京大学药学院, 北京 100083 本研究旨在探讨从国产尖吻蝮蛇蛇毒中 离的尖吻蝮蛇凝血酶( HCA ) 的止血作用及其作用机制以兔 全血凝固时间和小鼠断尾出血时间为指标评价 HCA 的促凝与止血作用; 以全血凝块溶解和纤维蛋白原裂解为指 标探讨 HCA 的止血作用机制结果表明, HCA 能显著缩短兔全血凝固时间和小鼠断尾出血时间, 有明显的剂量 依赖关系, 其作用与阳性对照药立止血( rep itilase)相似HCA 使纤维蛋白原 A 亚基裂解, 其诱导形成的血浆凝块 能在尿素溶液中溶解结论: HCA 通过水解纤维蛋白原的 A 亚基释放出A 肽, 形成纤维蛋白聚合体而产生止血作 用, 但不引起凝血因子ÚÓ的释放 尖吻蝮蛇凝血酶;止血作用; 纤维蛋白原 R996. 3; R619. 1 A H em ostatic E ffect of H em ocoagu lase A gk istrodon and ItsM echan ism 1 L B Hui-M i , LI Cha g-Li g , DONG Ji -Cha , ZHANG Cui-La , ZHOU Xia g-Ya Natio al I stitute of Radio log ica l Protectio a d Nuclear Safety, Chi ese Ce ter for D isease Co tro l a d Preve tio , Beiji g 100080, Chi a; 1 College o f Pharm aceutica l Scie ces, P eki g U iversity, Beiji g 100083, Chi a Abstract This study w as ami ed to i vestigate the hem ostatic effects of hem ocoagulase agk istrodo ( HCA ) a d its m echa ism. The procoagulative a d hem ostatic effects of HCA w ere evaluated by usi g rabit b lood coagu lati tmi e a d m ouse tail b leed i g tmi e; the m echa ism s of HCA hem ostatic effect w ere a alyzed by usi g rabb it blood clot lysis a d fibri oge lysis. The results show ed that HCA shorte ed the rabb it blood coagulatio tmi e a d the m ouse tail b leedi g tmi e sig ifica tly. The effects are early smi ilar to that of positive co trol ( rep tilase). HCA also i duced rabb it b lood clot lysis a d d irectly hydro lysed the A-chai of f ibri oge . It is co cluded that HCA exert its hem ostatic effects by