两种体色画眉笛雕的遗传多样性及分子标记.pdfVIP

第 28 卷 第 3 期 广东海洋大学学报 Vol.28 No.3 2008 年 6 月 Journal of Guangdong Ocean University Jun. 2008 两种体色画眉笛雕的遗传多样性及分子标记 刘 丽，刘楚吾，岳 文 (广东海洋大学水产学院，广东 湛江 524025) 摘 要：根据体侧纵带颜色差异，暂将画眉笛鲷（Lutjanus vitta ）分为黄带画眉笛鲷和褐带画眉笛鲷，应用RAPD 技术，选用 22 个引物对黄带画眉笛鲷与褐带画眉笛鲷进行遗传多样性及分子标记研究。结果表明，黄带画眉笛鲷 与褐带画眉笛鲷的遗传距离为 0.832 9 ，其多态位点比例（P ）分别为66.99%和 85.28% ，遗传多样性指数（H ）分 别为 0.161 7 和 0.289 3 ，种内遗传距离（D ）分别为0.243 5 和 0.431 9, 表明褐带画眉笛鲷的遗传多样性比黄带画眉 笛鲷丰富。共得到 OPP14-629bp 和 OPP14-674bp 等 11 个特异性分子标记，可用于两种笛鲷的鉴定。 关键词：画眉笛鲷；体色；遗传多样性；RAPD ；分子标记 中图分类号：Q75+Q959.483 文献标志码：A 文章编号：1673-9159（2008）03-0012-04 Genetic Diversity and Molecular Markers of Yellow and Brown Lutjanus vitta LIU Li ，LIU Chu-wu ，YUE Wen (Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China) Abstract: Yellow Lutjanus vitta were differentiated from the brown one according to the color of fish body strip. Genetic diversity and molecular markers of the two snappers were studied by random amplified polymorphic DNA（RAPD ）technique, and 22 random primers were analyzed in this experiment. The result showed that the genetic diversity of brown L. vitta was richer than that of yellow L. vitta. The inter-specific genetic distances between 2 Snapper Species was 0.832 9. The proportion of polymorphic loci （P ）was 66.99 % and 85.28 % respectively. The genetic diversity index was 0.161 7 and 0.289 3 in turn. The mean genetic distances （D ）of intra-species was 0.243 5 and 0.431 9 respectively. 11 molecular markers, such as OPP14-629bp and OPP14-674bp, which could be used for identifying 2 species of snappers were fo