1株紫色非硫光合细菌分离鉴定.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2008 ,36( 18) :7538 - 7539 责任编辑 张彩丽 责任校对 卢瑶 1 株紫色非硫光合细菌的分离鉴定 1,2 2 2 1* 2 * 刘芳 , 王敏 , 杨 慧, 孙军德 , 高俊莲 ( 1. 沈阳农业大学, 辽宁沈阳110 161;2 . 北京农业生物技术研究中心, 北京 100097) 摘要 [ 目的] 为紫 色非硫光合细菌的应用研 究打下基础 。[ 方法] 从杭州养鱼塘水样和底泥样品中分 离纯化到 1 株 紫 色非硫光合细菌 菌株, 通过对其细胞形态观察 、活细胞色素吸收光谱测定及生理生化特征研 究等进行分类鉴定。[ 结果] HZ- 1 菌株可在 光照厌氧或黑暗 好氧条件下生长; 菌体呈短杆状, 稍 弯, 为革兰氏阴性菌株; 含有细菌叶绿素a 和类胡萝 卜素; 接触 酶试验、吲哚试验、硝 酸盐还原试验 阳 性, 硫化氢生成试验、明胶液化试验 阴性。[ 结论] 通过对分 离纯化的紫 色非硫光合细菌菌株 的分类鉴 定, 判 断菌株 HZ- 1 为沼泽红假单 胞菌。 关键词 紫 色非硫光合细菌; 分 离鉴定; 菌株 中图分类号 S182 文献标识码 A 文章编号 0517 - 6611(2008) 18 - 07538 - 02 Isolation and Identification on A Strain of Purple Non- Sulfur Photosynthetic Bacteria LI U Fang et al ( Shenyang Agricultural University ,Shenyang ,Liaoning 110 161) Abstract [ Obj ective] The research ai med to provide a basis for the application of purple non-sulfur photosynthetic bacteria .[ Method] A strai n purple non- sulfur photosynthetic bacteria was isolated and purified fromthe water sample and sedi ment of fish pond of Hangzhou .The classification and identifica- tion of purple non- sulf ur photosynthetic bacteria was carried out by the observation of cell morphology ,the cell coloring matter absorption spectrumdeter mi- nation and the study of physiological and bioche mical characteristics .[ Results] The HZ- 1 strain could grow under the condition of illuminated anaerobic or dark aerobic .The strain was gramnegative and the cell of the strain was in the shape of short and slightly curved rod . The cells contai ned bacteria chloro- phyll a and li ke- carotene .The contact enzyme test ,i ndole production test and nitrate reductiontest was positive reaction and the hydrogen sulfide for mation test and gelatin li