宫颈鳞状细胞癌脆性组氨酸三体FHIT蛋白表达缺失及其意义.pdfVIP

· 835· 肿瘤防治研究 2004 年第 31 卷第 9 期 宫颈鳞状细胞癌脆性组氨酸三体 FHIT 蛋白 表达缺失及其意义 赵 　坡 ,侯 　宁 ,曹友俊 LossofFra gileHistidineTriadProteinEx pressioninCervicalS quamousCellCarcinoma ZHAOPo,HOUNin g,CAOYou jun Department of Patholo gy , PLA General Hospital , Beijin g 100853 , China Abstract:Objective 　Toinvesti gatetheex pressionoffra gilehistidinetriad gene protein,FHITandthe possi blerelationshi pbetweenFHITex pressionandclinico patholo gicalindicesincervicals quamouscellcarcinoma. Methods 　Detectin gFHIT proteinex pressionin100cervicals quamouscellcarcinomas,83 paraneoplastictis suesand10normalcervicaltissuesandanal ysingitsrelationshi ptohistolo gical gradeandclinicalsta geb ySP immunohistochemicalmethod. Results　Therewasasi gnificantdifferenceinFHITex pressionbetweencarci nomasand paraneoplasticat ypicalh yperplastictissuesornormalcervicals quamouse pithelia ( P = 0.000 ) . Carcinomaswithdecreasedex pressionofFHITwerecorres pondin gtohistolo gical grade ( P = 0.001 ) andclin icalsta ge ( P = 0.001 ) . Conclusion　Thedecreasedex pressionofFHITma ybeassociatedwiththecarcino genesis,decreasin gdifferentiationandinvasivebehaviorofcervicals quamouscellcarcinoma.Itissu ggested thatthelossofFHITex pression playsanim portantroleinthedevelo pmentand pro gressionofthetumor,and thusma ybecomeanewad junctdia gnosticand pro gnosticmarkerincervicals quamouscellcarcinoma. Keywords: Cervicalcancer;FHIT;Immunohistochemistr y 摘 　要 : 目的　探讨宫颈鳞状细胞癌中FHIT 蛋白表达与临床病理的关系 。方法 　采用 FHIT 蛋白抗体 和免疫组化 SP 法检测 100 例宫颈鳞状细胞癌 、83 例癌旁不典型增生组织和 10 例正常宫颈组织中 FHIT 表达并分析其与组织学分级和临床分期的关系 。结果 　FHIT 蛋白表达的规律为从正常 →癌旁 ( ) →鳞癌 ,其表达逐渐减低 。癌组织与癌旁及正常组织比较 ,差异均非常显著 P = 0.000 。FHIT 蛋白表 ( ) 达也随鳞癌组织学分级及