代谢组学技术在针刺研究设计中质量控制_周思远.pdfVIP

# 836# 辽宁中医杂志 2010年第 37 卷第 5期 1 2 2 2 , , , ( 11 成都中医药大学针灸推拿学院硕士研究生, 四川成都 610075; 21 成都中医药大学针灸推拿学院, 四川成都 610075) : 代谢组学技术作为系统生物学的重要组成部分已被广泛地应用于多 个领域, 但 结果易受多种因素的影 响近来本课题组率先将代谢组学这 一新兴技术引入针刺临床研究领域, 并针对人体代谢物易受外界刺激干扰的特点, 参照文献设计了该技术在针刺研究中的质量控制方法, 包括严格筛选受试对象, 适当控制受试对象的饮食运动昼夜节 律, 规范样本的采集处理和保存以及统一针刺刺激量等措施, 以便减少干扰, 突出针刺作用该方法有助于提高代谢组 学技术应用于针刺领域的研究质量 : 代谢组学; 针刺研究; 质量控制 : R2- 03 : A : 1000- 1719( 2010) 05- 0836- 03 Quality Control ofM etabonom ics in Acupuncture Research Design 1 2 2 2 ZH O U S i-yuan , W U Q iao-f eng , LI Y ing , YU Shu-g uang ( 1. M as ter P os tg raduate in C o eg e of A cup uncture and T uina, Cheng du University of TCM , Cheng du 610075, S ichuan, Ch ina; 2. Co eg e of A cup uncture and T uina, Chengdu Un iv er sity of T CM, Cheng du 6 10075, S ichuan, Ch ina ) Abstract: A s a part of s stem s biolog , m etabonom ics has been w idel used in m an areas. Recentl, our research group has taken the lead to in troduce m etabonom ics, an prom ising technolog , into the field of clin ical acupuncture research. B ecause the outcom e ofm etabonom ics would be influenced b variet factors such as spic food and heav exercise as literature reported, w e took som e measures to control the qualit of thewhole stud and thus to emphas ize acupuncture effects. Thesem ethods include re- strictions of subject s diet, ex ercise and diurnal rh thm, standardizing collecting, processing and preservation of sam ples and unif- ing the am ounts of acupuncture stmi ulations, etc. Therefore, m an possib le metabolic