wwmicroRNA在胚胎干细胞中的表达与作用.pdfVIP

HEREDITAS (Beijing) 2008 12 , 30(12): 1545―1549 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.01545 microRNA 王世华, 边春景, 赵春华 , 100005 : 胚胎干细胞是一类具有自我更新能力和多向分化潜能的细胞, 其自我更新和多向分化过程都在遗传和表 观遗传的严格调控下进行的。越来越多的研究表明microRNA 也在这一过程中发挥重要的作用。microRNA 是 一类内源性的非编码RNA, 能够通过与靶mRNA 特异性的结合而导致靶mRNA 降解或抑制其翻译, 从而对基 因进行转录后调控。文章就microRNA 在胚胎干细胞中的表达及其作用的研究进展做一综述。主要讨论一些在 胚胎干细胞中特异性表达的 microRNA, 以及这些microRNA 对胚胎干细胞自我更新和未分化状态的维持和继 续分化增殖的调控作用。 : 胚胎干细胞; microRNA; 分化 Expression and function of microRNA in embryonic stem cell WANG Shi-Hua, BIAN Chun-Jing, ZHAO Chun-Hua Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100005, China Abstract: Embryonic stem cells are the cells that have the ability both to self-renew and to differentiate into all the mature cell types in an adult. Both of these processes are tightly regulated by genetic and epigenetic mechanisms. Increasing evi- dence indicates that a class of single-stranded non-coding RNA, known as “microRNAs”, also plays a critical role in this process. MicroRNA can bind to target mRNAs by specific base pairing, then degrade mRNAs or inhibit protein translation. Therefore, they can participate in post-transcriptiona1 regulation. Recently, scientists began to study the effect of microRNA on embryonic stem cell and found that some microRNAs are specifically expressed and form an intricate network of mi- croRNAs, regulating key transcription factors and other genes. This review focuses on the expression of microRNA in em- bryonic stem cell and their functions. We discuss some microRNA that are specifically expressed in embryonic stem cell and their regulating effect on self-renewal and differentiation. Keywords: embryonic stem cell; micr