SSR分子标记开发策略与评价.pdfVIP

遗 　传 HEREDITA S ( Beijing) 26 (5) :763～768 ,2004 专论与综述 S S R 分子标记开发策略及评价 张增翠 ,侯喜林 (南京农业大学作物遗传与种质创新国家重点实验室 ,南京 2 10095) 摘 　要 :SSR 标记以其多态性丰富、提供遗传信息多、操作便利并在基因组中分散分布等优点已成为最受人们欢迎 的分子标记之一 ,在许多领域广泛应用 。但 SSR 标记的主要缺点是首先要从该物种中获取重复序列两侧的序列信 息 ,并设计引物 ,而后才能被利用 。综述了几种有代表性的 SSR 标记开发策略 , 旨在为各物种 SSR 标记的开发提供 参考信息 。 关键词 :SSR ;开发 ;策略 中图分类号 :Q943 　　　文献标识码 :A 　　　 文章编号 :0253 - 9772 (2004) 05 - 0763 - 06 S t r a t e gi e s f o r D e v e l op m e n t of S S R M ol e c u l a r Ma r k e r s ZHAN G ZengCui , HOU XiLin ( National Key Laboratory of Crop Genetic s and Germpla sm Enhancement ,Nanjing Agricultural Univer sity ,Nanjing 2 10095 , China) A b s t r a c t : SSR molecular marker s have be en use d widely in many genetic studie s and become one of the mo st popular molecular marker s due to their high polymorphi sm ,abundant informativene ss ,convenience of a ssay by PCR and di stri bution throughout the genome . The major drawback of SSR molecular marker s i s that their primer s ne e d to be de signe d according to the i solat e d sequence flanking the SSR from sp ecie s that are being examine d for the fir st time . The aim of the pre sent p ap er i s to review the several repre sent ative methods of SSR i solation de scribe d in the lit erature and pro vide useful information in making appropriat e choice s among the large number of currently available options. Ke y w or d s : SSR ; development ; strat egy ( ) [3 ] 　　简单序列重复 SSR , Simple Sequence Repeat 生物基因组 中 , 因此 已广泛应用于遗传 图谱构 ( Tautz D 1989) [ 1 ] ,又称微