基于基因芯片对微生物基因功能和群落结构分析的硫化矿生物浸出分析.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, June 25; 24(6): 968-974 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 基于基因芯片对微生物基因功能与群落结构分析的硫化 矿生物浸出分析 1, 2 1, 2 1, 2 申丽 , 刘学端 , 邱冠周 1 中南大学资源加工与生物工程学院, 长沙 410083 2 教育部生物冶金重点实验室, 长沙 410083 摘 要: 生物冶金技术因具有流程短、成本低、环境友好, 且特别适合处理低品位、复杂、难处理的矿产资源等优点, 已经成为研究热点。然而由于缺少高效菌种以及不能对浸矿体系微生物进行定量分析, 难以对浸矿工艺参数和微生物种 群进行优化调控, 从而导致硫化矿生物浸出速度慢、浸出率低。随着基因芯片、菌种保存技术的发展, 这些难题在逐一 被解决。对近年来针对硫化矿浸出过程微生物的基因功能与群落结构分析的研究进行了概述, 将帮助我们更好地了解基 因组学与生物冶金技术结合的重要作用。 关键词: 硫化矿, 生物浸出, 生物冶金, 基因组学, 基因芯片 Gene Function and Microbial Community Structure in Sulfide Minerals Bioleaching system Based on Microarray Analysis Li Shen1, 2, Xueduan Liu1, 2, and Guanzhou Qiu1, 2 1 School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China 2 The key Lab of Biometallurgy, Ministry of Education , Changsha 410083, China Abstract: Biohydrometallergy technology received more and more attention because of its simple process, low cost and kind to environment, especially in dealing with low-grade and complex minerals. However, it is difficult to optimize microorganism species and process parameters in bioleaching procedure because of the lack of suitable bacteria and quantitative analysis methods at micro-level for bioleaching system. This has resulted in the low efficiency and poor yield of the target metal in bioleaching. With the development of microarray and bacteria con