姜黄素抗兔动脉粥样硬化作用的研究.pdfVIP

200 1 2 Zhejiang JITCWM (Vol1 1 No1 2 200 ) 67 # # 姜黄素抗兔动脉粥样硬化作用的研究 赵志光 谢丽微 王宗敏 325027 林梅瑟 ICU : 研究姜黄素抗兔动脉粥样硬化( AS) 的作用机制: 30只 性日本大耳白家 兔, 其中给予高脂饲料喂食 2 个月, 复制 AS模型家兔 20 只, 随机分成治疗和对照两组, 各 10 只 均予基础饲料喂养, 治疗组每天给予姜黄素( 200mg /kg)灌胃, 对照组每天给予生理盐水灌胃, 共治 疗 1 个月治疗后处死家兔, 观察两组主动脉粥样斑块面积比; 取主动脉弓做病理切片; 免疫组织 化学检测主动脉壁主动脉基质金属蛋白酶 9(MMP-9)和核转录因子-JB( NF-JB)的表达: 治 疗组斑块面积/主动脉面积比低于对照组(P 0. 05) 免疫组化显示, 治疗组MMP-9NF-JB 阳性 细胞表达率明显低于对照组, 分别为[ ( 16. 5 ? . 0)% vs ( 3 . 5 ? 13. 6)% , P 0. 05; ( 22. 61 ? 7. 95)% vs ( 42. 2 ? 19. 34)%, P 0. 05] : 姜黄素可以显著降低动脉粥样硬化家兔主动脉 壁MMP-9NF-JB的表达, 抑制动脉粥样硬化的形成 兔 动脉粥样硬化 姜黄素 核因子 JB 基质金属蛋白酶 9 免疫组织化学 The Effect ofCurcum in on R esisting A therosclerosis in Rabbits Zhao Zhiguang, et al. D ep ar tm ent of Patho logy, The Second Aff ilia ted H osp ital of Wenzhou M ed ical Co llege, Wenzhou( 325027 , China Abstract Objective: To investigate the effect of curcum in on resisting atherosclerosis in the aorta atheroscleroticRabb its. M ethods: Th irtym ale JapaneseW hite rabb itswere fedw ith a h igh cholesterol d iet for tw o consecutivem onths. F inally, 20 rabb itswere estab lished atheroscleroticm odels. They are random ly d ivided into two groups: the control group ( 10 an mi als) and the treatm ent group ( 10 an mi als) . Both groupsw ere fed w ith a standard diet in the follow ingm onth. Additionally, the treatm ent group received an oral curcum in at a dose of 200mg/Kg body w t and the control group received an oral curcum in- free sodium ch loride solution daily during expermi ent. At the end of therapy period, all anmi als w ere k illed. Entire aorta w as rapidly d issected ou