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Influence of electric current on bacterial viability in wastewater treatment V. Wei a,*, M. Elektorowicz b, J.A. Oleszkiewicz Department of Civil Engineering, University of Manitoba, 15 Gillson St., Winnipeg, Canada R3T 5V6 Department of Building, Civil and Environmental Engineering, Concordia University, 1455 de Maisonneuve Blvd. West, Montre′al Canada H3G 1M8 Article info Article history: Received 26 April 2011 Received in revised form 24 June 2011 Accepted 5 July 2011 Available online 19 July 2011 Keywords: Electric current、Wastewater treatment、Electrochemistry、Microorganisms、Bacterial viability Abstract（摘要） Minimizing the in?uence of electric current on bacterial viability in the electro technologies such as electrophoresis and electrocoagulation is crucial in designing and operating the electric hybrid wastewater treatment system. In this study the biomass from a membrane bioreactor (MBR) was subjected to constant direct current and the bacterial viability was monitored against electrical intensity, duration as well as the spatial vicinity related to the electrodes. It was found that the bacterial viability was not significantly affected (less than 10% of death percentage) when the applied electric current density (CD) was less than 6.2 A/㎡ after 4 h. The percentage of live cell dropped by 15% and 29% at CD of 12.3 A/㎡ and 24.7 A/㎡, respectively. The pH of electrolytic biomass fluid has shifted to alkaline (from nearly neutral to around pH 10) at CD above 12.3 A/㎡, which could have been the contributing factor for the bacterial inactivation. The temperature change in the electrolytic media at all current densities during 4 h of experiment was less than 2℃, thus temperature effects were negligible. Bacteria experienced different micro-environments in the electrochemical reactor. Bacterial cells on the cathode surface exhibited highest death rate, whereas bacteria outside the space between electrodes were the least affected. It was concluded that in an electro-te