改良差异裂解法结合硅珠法提取混合斑精子DNA.pdfVIP

· 50 · JoumalofForensicMedicine，February2014，Vol-30，No．1 改良差异裂解法结合硅珠法提取混合斑精子DNA 韩海军 ．一，张玉红 ，杨 敏 -一，伊 海 --，杨耕野 ，贾东涛 ，卢大儒 (1．复旦大学生命科学学院，上海 200433；2．南通市公安局物证鉴定所 ，江苏 南通 226007) 摘 要：目的 采用改 良差异裂解法结合硅珠法提取混合斑中精子DNA，并评价其应用价值 。 方法 收集 52例经常规差异裂解法检验含有女性分型的混合斑检材，采用改 良差异裂解法结合硅珠法提取精子细胞 DNA，IdentifilerMT试剂盒进行PCR扩增检验。并将常规差异裂解法结果作为对照。 结果 52例混合斑检材 中，采用改良差异裂解法结合硅珠法检出单一男性精子成分有38例 ，男性分型检出率达到98．08％。 结论 改 良差异裂解法结合硅珠法适合提取混合斑中精子DNA。 关键词：法医遗传学；混合斑：差异裂解法；硅珠法 中图分类号 ：DF795．2 文献标志码 ：A doi：10．3969／j．issn．1004—5619．2014．01．012 文章编号 ：1004—5619(2014)叭一0050—02 Extraction of Sperm DNA from M ixed Stain by the M odified DifierentialLysis M ethod Combined with Silicon Bcad M ethod HANHai-ju#,2,ZHANGYu—hong2,YANGM 0．YIHail，2YANGGeng-ye2,JIADong-tao2,LUDa—IU1 1．SchoolofLireSciences，Fudan University,Shanghai2oo433，China；2．InstituteofForensicScience， NantongPublicSecurityBureau,Nantong226007,China) Abstract：0b 仃 DNA ixed stain by themodified differential1ysismethod ．iectiveToex actsperm from m ． combined with siliconbeadmethod and to evaluate itsapplication value． M ethodsFiftytwo mixedstains containing female STR genotypesdetected by differentiallysismethod were collected．The spemr DNA was extracted by the modified method combined with silicon bead method，then genotyped with the IdentifilerMT Kit．and compared withtheresultsofgenotypingbyhte conventionaldifferential1ysismethod ascontro1．Results Ofthe 52 samples．38 sampleswith sole male STR genotypesin alllOCiwere de— tected．The detection rate ofmale STR genotypeswas98．08％ through the modified method combined with silicon bead method．Conclusion The modified difierential1ysismethod combined with silicon bead method canbeused in extraction ofsperm DNA r