毒死蜱高效降解酶保护剂配方优化及稳定性.pdf

2009,42(1):136-144 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.01.017 510642 · · Optimization of Enzyme Protectant of Chlorpyifos-Biodegradation Fungi and Its Stability ZHONG Guo-hua, HE Yue, LIU Xuan-qing, HU Mei-ying, GAO Yan (Lab of Insect Toxicology, South China Agricultural University, Guangzhou 510642) Abstract:ObjectiveThe objective of this study was to optimize the composition of enzyme protectant of chlorpyifos- biodegradation fungi and determine its stability.MethodBased on the screening of chlorpyrifos biodegrading fungal strain, Cladosporium cladosporioides (the ID of fungi preservation CCTCCM207111. Accession number of ITS sequence analysis Genebank EF405864), extracellular enzymes were extracted. Applying the composite rotatable design, a model of the optimal mixture protect agents was established. After the optimal composition was validated, the enzyme preparation storage experiment was tested to determine the stability of biodegradation by high performance liquid chromatography analysis. Result The results showed that the optimal composition was 0.70% NaCl, 0.35% glycine, 0.03% sodium benzoate, 0.17% Cosmetic, and 8.38% glycerol. When 9.90 mg·L-1 of final concentration of emzyme protein was added into the 50 mg·L-1 of chlorpyrifos, results of HPLC analysis after biodegradation 10 min indicated that the degradation rate of the enzyme preparations was more than 80% after storage 5 months under the temperature from 20 to 50 and the light almost has no effect on the degradation rate. Conclusion It is concluded that an economical and stable enzymic protectant of chlorpyifos-biodegradation fung