水通道蛋白亚型1_5在鼠鼻粘膜中表达及意义.pdf

! 528 ! Chin J Lab Diagn,April,2007, Vol 11, No. 4 : 1007- 4287(2007) 04- 0528- 03 1- 5 1 2 3 1 1 朱建国, 崔 航 , 赵 丹, 雷 霏 , 赵小冬 ( 1. - , 130033; 2. 2004 , 130021; 3. , 130021) : (water channel protein;Aquaporin AQ ) 20 Wistar , 3% , ,4% , 12345 AQ 14 11000,AQ 3 1300,AQ 5 1400 AQ 2 150 ,AQ 1 ,AQ 3 ,AQ 4 , AQ 5 , 1345 , , : ; ; : Q591.2 :A Localization anddistributionofAquaporin 1- 5 inratnasal mucosa ZH UJianguo, CUI Hang , ZHA ODan, et al . ( 1. Depart ment of Otorhinolaryngology, Head and Neck urgery, ChinaJapan union Hospital, Jilin University, Changchun 130031, China; 2. Department of Pathop hysiology Jilin University 130021) Abstract:Objective To determine the localization and distribution of Aquaporin 1- 5in rat nasal mucosa.We discuss the de velopment of the water disorder inducing disease in this tissue.Methods Samples of ratnasal mucosa were removed from 20 grown Wistar rats.The samples were fixedwith paraformaldehyde and embedded in paraffin. Immunohistochemistry of nasal mucous sections were performedwith polyclonal antiAQ 1- 5 antibodies. Results Thedilution of AQ 1,4was 11000 ,AQ was1300.AQ was 1 3 5 400, Significantly express in the basal membrane, ducfsand spasn in the glands. Conclusion Aquaporin 1345 express in the dif ferent Sites in nasal mucosa.Abnormal expression of and of them may resalts in the water metabolism disorders. ey words:water channel protein nasal mucosa mmunohistochemistry ( Chin J Lab Diagn, 2007, 11: 0528) , , 6 m, (Aqua 40 , , porins, AQ