纳米硒对波尔山羊睾丸发育的影响.pdfVIP

纳米硒对波尔山羊睾丸发育的影响 1 1 1 1 2 施力光 杨茹洁 岳文斌 * 张春香 雷晓峰 (1. 山西农业大学动物科技学院，山西 太谷 030801 ； 2. 山西农业大学试验基地黎城县种羊场 山西 黎城 047600) 摘 要：为了研究日粮中添加纳米硒对公羊睾丸发育的影响，试验选用体重、日龄接近的 20 只波尔山羊公羔羊随机分两组，一组日粮中添加0.3 mg/kg 的纳米硒( 以硒计) ，另一组饲 喂基础日粮，预试2 周，试验期每月测量睾丸周径、长度和高度，每组阉割一只公羔，按照 常规方法制作石蜡切片，观察睾丸组织结构。结果显示，3 月龄时纳米硒组睾丸间质组织增 生发育，4 月龄时纳米硒组睾丸曲精细管中精原细胞开始发育分化；5 月龄时曲精细管中可 见大量的精子，而对照组间质组织以及曲精细管发育、精母细胞发育分化较纳米硒组缓慢。 试验表明，日粮中添加一定浓度的纳米硒能够促进睾丸间质组织及曲精细管的发育，促进精 母细胞的分化。 关键词：纳米硒，波尔山羊，睾丸组织，精子 Effect of Nano-Selenium on Testicular Growth and Development in Boar Goat 1 1 1 1 2 Shi Li-guang , Yang Ru-jie , Yue Wen-bin *, Zhang Chun-xiang , Lei Xiao-feng (1. College of Animal Science and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 2. Licheng sheep Breeding Base of Shanxi Agricultural University ， Licheng Shanxi 047600，China) Abstract: An experiment was conduct to study the effect of nano-Se on testicular growth and development in Boar Goat. Total 20 goats were randomly divided into 2 groups, and fed basal diet or supplementation 0.3 mg/kg nano-Se (as Se content). After 2 weeks of pretrial, the testicular length, height and girth were measured for every month, and castratsd one randomly. One of the testis was made the microscopic section, and observed the testicular microstructure. The results showed that the interstitial tissue was observed in 3 month-old, and spermatogonia began developed in convoluted seminiferous tubules in 4 month, the spermatozoa and spermatids were observed in 5 month of nano-Se group. Compared with the supplementation group, the