七叶鬼灯檠总酚微波辅助提取工艺优化及其DPPH·自由基清除能力的分析.pdfVIP

七叶鬼灯檠总酚微波辅助提取工艺优化及其DPPH·自由基 清除能力分析 孙婷 肖亚萍 王喆之* 药用资源与天然药物化学教育部重点实验室，西北濒危药材资源开发国家工程实验室， 陕西师范大学生命科学学院，西安 710062 摘要：探索七叶鬼灯檠总酚微波辅助提取最佳工艺条件及其DPPH·自由基清除能力。通过单因素试验 和正交试验分别考察了微波辅助下超声波提取功率、乙醇浓度、料液比以及提取时间等不同因素对七叶 鬼灯檠总酚提取率的影响，并考察了总酚对DPPH·自由基的清除能力。七叶鬼灯檠总酚最佳微波辅助 提取工艺为：提取功率500W、乙醇浓度40％、料液比1:30、提取时间8min。在最佳提取工艺条件下， 总酚得率为8.61％，其清除DPPH·自由基的EC50为12.20±1.60μg/mL。本研究建立了一种七叶鬼 灯檠总酚微波辅助提取最佳工艺，同时，证明其总酚提取物具有较强的清除自由基的能力。 关键词：七叶鬼灯檠;微波辅助提取;总酚;DPPH·自由基 StudyonExtractionTechnologyofTotalPolyphenolfromRhizomesof RodgersiaaesculifoliaandItsScavengingCapacityAgainsttheDPPH·Radical Sun-TingXiao-YapingWang-Zhezhi* KeyLaboratoryofMinistryofEducationforMedicinalResourcesandNaturalPharmaceutical Chemistry;NationalEngineeringLaboratoryforResourceDevelopingofEndangeredChineseCrude DrugsinNorthwestofChina;CollegeofLifeSciences,ShaanxiNormalUniversity,Xian,Shaanxi 710062,China Abstract::TochoosetheoptimumextractiontechnologyoftotalpolyphenolfromrhizomesofRodgersia aesculifoliabymicrowave. Thesinglefactorexperimentsandorthogonaltesteswerecarriedoutto investigatetheoptimumextractionconditions(ethanolconcentration,extractiontime,materialliquid, ultrasonicpower)oftotalpolyphenolfromrhizomesofR.aesculifolia. Itsscavengingcapacityagainstthe DPPH.radicalwasalsodetermined. Optimumextractionconditionsweredeterminedasfollows:microwave power500W,time8rmins,40％ EtOHasextractingsolvent,stockratio1:30(μg/mL,mass:volume).The extractiverateoftotalpolyphenolreached8.61％.IC50ofscavengingDPPH·freeradicalsoftotalpolyphenol was12.20± 1.60μg/mLattheoptimumparameters.Thispapersuppliedanoptimummicrowaveextraction processoftotalpolyphenolfromrhizomesofR.aesculifolia.Theseextractionshavestrongscaveniging capacityagainsttheDPPH·radical. Keywords:RodgersiaaesculifoliaBatalin;microwave-assistedextractionmethod;totalpolyphenol; DPPH·freeradicals ~U)tJZU30,． 65℃，提马 垄耿提愀列Jf 匕叶鬼灯檠^ 1:40